Click chemistry to construct fluorescent oligonucleotides for DNA sequencing
Click chemistry 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with singlebase resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.
- Publisher
- AMER CHEMICAL SOC
- Issue Date
- 2003-01
- Language
- English
- Article Type
- Article
- Keywords
CUCURBITURIL; CYCLOADDITION; CATALYSIS; PRIMERS
- Citation
JOURNAL OF ORGANIC CHEMISTRY, v.68, no.2, pp.609 - 612
- ISSN
- 0022-3263
- Appears in Collection
- CBE-Journal Papers(저널논문)
- Files in This Item
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