DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, Peng | ko |
dc.contributor.author | SEO, TAE SEOK | ko |
dc.contributor.author | Beyor, Nathaniel | ko |
dc.contributor.author | Shin, Kyoung-Jin | ko |
dc.contributor.author | Scherer, James R. | ko |
dc.contributor.author | Mathies, Richard A. | ko |
dc.date.accessioned | 2009-05-06T02:22:28Z | - |
dc.date.available | 2009-05-06T02:22:28Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-03 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.79, no.5, pp.1881 - 1889 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/8847 | - |
dc.description.abstract | A portable forensic genetic analysis system consisting of a microfluidic device for amplification and separation of short tandem repeat (STR) fragments as well as an instrument for chip operation and four-color fluorescence detection has been developed. The microdevice performs polymerase chain reaction (PCR) in a 160-nL chamber and capillary electrophoresis (CE) in a 7-cm-long separation channel. The instrumental design integrates PCR thermal cycling, electrophoretic separation, pneumatic valve fluidic control, and four-color laser excited fluorescence detection. A quadruplex Y-chromosome STR typing system consisting of amelogenin and three Y STR loci (DYS390, DYS393, DYS439) was developed and used for validation studies. The multiplex amplification of these 4 loci with 35 PCR cycles followed by CE separation and 4-color fluorescence detection was completed in 1.5 h. All the amplicons can be detected with a limit of detection of 20 copies of male standard DNA in the reactor. Real-world forensic analyses of oral swab and human bone extracts from case evidence were also successfully performed. Mixture analysis demonstrated that a balanced profile can be obtained even at a male-to-female template ratio of 1:10. The successful development and operation of this portable PCR-CE system establishes the feasibility of rapid point-of-analysis DNA typing of forensic casework, of mass disaster samples or of individuals at a security checkpoint. | - |
dc.description.sponsorship | Grant 2004-DN-BX-K216 awarded by the NAtional Institute of Justice | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | DNA AMPLIFICATION | - |
dc.subject | GENETIC-ANALYSIS | - |
dc.subject | MICROFLUIDIC DEVICES | - |
dc.subject | ANALYSIS SYSTEMS | - |
dc.subject | PCR | - |
dc.subject | SAMPLES | - |
dc.subject | BIOPROCESSOR | - |
dc.subject | MICRODEVICE | - |
dc.subject | EXTRACTION | - |
dc.subject | MULTIPLEX | - |
dc.title | Integrated portable polymerase chain reaction-capillary electrophoresis microsystem for rapid forensic short tandem repeat typing | - |
dc.type | Article | - |
dc.identifier.wosid | 000244497300012 | - |
dc.identifier.scopusid | 2-s2.0-33847676392 | - |
dc.type.rims | ART | - |
dc.citation.volume | 79 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1881 | - |
dc.citation.endingpage | 1889 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/ac061961k | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | SEO, TAE SEOK | - |
dc.contributor.nonIdAuthor | Liu, Peng | - |
dc.contributor.nonIdAuthor | Beyor, Nathaniel | - |
dc.contributor.nonIdAuthor | Shin, Kyoung-Jin | - |
dc.contributor.nonIdAuthor | Scherer, James R. | - |
dc.contributor.nonIdAuthor | Mathies, Richard A. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DNA AMPLIFICATION | - |
dc.subject.keywordPlus | GENETIC-ANALYSIS | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICES | - |
dc.subject.keywordPlus | ANALYSIS SYSTEMS | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | SAMPLES | - |
dc.subject.keywordPlus | BIOPROCESSOR | - |
dc.subject.keywordPlus | MICRODEVICE | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | MULTIPLEX | - |
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