Integrated portable polymerase chain reaction-capillary electrophoresis microsystem for rapid forensic short tandem repeat typing

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dc.contributor.authorLiu, Pengko
dc.contributor.authorSEO, TAE SEOKko
dc.contributor.authorBeyor, Nathanielko
dc.contributor.authorShin, Kyoung-Jinko
dc.contributor.authorScherer, James R.ko
dc.contributor.authorMathies, Richard A.ko
dc.date.accessioned2009-05-06T02:22:28Z-
dc.date.available2009-05-06T02:22:28Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2007-03-
dc.identifier.citationANALYTICAL CHEMISTRY, v.79, no.5, pp.1881 - 1889-
dc.identifier.issn0003-2700-
dc.identifier.urihttp://hdl.handle.net/10203/8847-
dc.description.abstractA portable forensic genetic analysis system consisting of a microfluidic device for amplification and separation of short tandem repeat (STR) fragments as well as an instrument for chip operation and four-color fluorescence detection has been developed. The microdevice performs polymerase chain reaction (PCR) in a 160-nL chamber and capillary electrophoresis (CE) in a 7-cm-long separation channel. The instrumental design integrates PCR thermal cycling, electrophoretic separation, pneumatic valve fluidic control, and four-color laser excited fluorescence detection. A quadruplex Y-chromosome STR typing system consisting of amelogenin and three Y STR loci (DYS390, DYS393, DYS439) was developed and used for validation studies. The multiplex amplification of these 4 loci with 35 PCR cycles followed by CE separation and 4-color fluorescence detection was completed in 1.5 h. All the amplicons can be detected with a limit of detection of 20 copies of male standard DNA in the reactor. Real-world forensic analyses of oral swab and human bone extracts from case evidence were also successfully performed. Mixture analysis demonstrated that a balanced profile can be obtained even at a male-to-female template ratio of 1:10. The successful development and operation of this portable PCR-CE system establishes the feasibility of rapid point-of-analysis DNA typing of forensic casework, of mass disaster samples or of individuals at a security checkpoint.-
dc.description.sponsorshipGrant 2004-DN-BX-K216 awarded by the NAtional Institute of Justiceen
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherAMER CHEMICAL SOC-
dc.subjectDNA AMPLIFICATION-
dc.subjectGENETIC-ANALYSIS-
dc.subjectMICROFLUIDIC DEVICES-
dc.subjectANALYSIS SYSTEMS-
dc.subjectPCR-
dc.subjectSAMPLES-
dc.subjectBIOPROCESSOR-
dc.subjectMICRODEVICE-
dc.subjectEXTRACTION-
dc.subjectMULTIPLEX-
dc.titleIntegrated portable polymerase chain reaction-capillary electrophoresis microsystem for rapid forensic short tandem repeat typing-
dc.typeArticle-
dc.identifier.wosid000244497300012-
dc.identifier.scopusid2-s2.0-33847676392-
dc.type.rimsART-
dc.citation.volume79-
dc.citation.issue5-
dc.citation.beginningpage1881-
dc.citation.endingpage1889-
dc.citation.publicationnameANALYTICAL CHEMISTRY-
dc.identifier.doi10.1021/ac061961k-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorSEO, TAE SEOK-
dc.contributor.nonIdAuthorLiu, Peng-
dc.contributor.nonIdAuthorBeyor, Nathaniel-
dc.contributor.nonIdAuthorShin, Kyoung-Jin-
dc.contributor.nonIdAuthorScherer, James R.-
dc.contributor.nonIdAuthorMathies, Richard A.-
dc.type.journalArticleArticle-
dc.subject.keywordPlusDNA AMPLIFICATION-
dc.subject.keywordPlusGENETIC-ANALYSIS-
dc.subject.keywordPlusMICROFLUIDIC DEVICES-
dc.subject.keywordPlusANALYSIS SYSTEMS-
dc.subject.keywordPlusPCR-
dc.subject.keywordPlusSAMPLES-
dc.subject.keywordPlusBIOPROCESSOR-
dc.subject.keywordPlusMICRODEVICE-
dc.subject.keywordPlusEXTRACTION-
dc.subject.keywordPlusMULTIPLEX-
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