DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, CCY | ko |
dc.contributor.author | SEO, TAE SEOK | ko |
dc.contributor.author | Li, ZM | ko |
dc.contributor.author | Ruparel, H | ko |
dc.contributor.author | Ju, JY | ko |
dc.date.accessioned | 2009-05-06T02:14:28Z | - |
dc.date.available | 2009-05-06T02:14:28Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-05 | - |
dc.identifier.citation | BIOCONJUGATE CHEMISTRY, v.14, no.3, pp.697 - 701 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | http://hdl.handle.net/10203/8845 | - |
dc.description.abstract | We report the site-specific fluorescent labeling of DNA using Staudinger ligation with high efficiency and high selectivity. An oligonucleotide modified at its 5' end by an azido group was selectively reacted with 5-[(N-(3'-diphenylphosphinyl-4'-methoxycarbonyl)phenylcarbonyl)aminoacetamido]fluorescein(Fam) under aqueous conditions to produce a Fam-labeled oligonucleotide with a high yield (similar to90%). The fluorescent oligonucleotide was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Because of the relatively high yield of the Staudinger ligation, simple purification of the product by size-exclusion chromatography and desalting is sufficient for the resulting fluorescent oligonucleotide to be used as a primer in a Sanger dideoxy sequencing reaction to produce fluorescent DNA extension fragments, which are analyzed by a fluorescent electrophoresis DNA sequencer. The results indicate that the Staudinger ligation can be used successfully and site-specifically to prepare fluorescent oligonucleotides to produce DNA sequencing products, which are detected with single base resolution in a capillary electrophoresis DNA sequencer using laser-induced fluorescence detection. | - |
dc.description.sponsorship | The National Science Foundation Biophotonics Partnerdhip Initiative Grant 86933 and Sensing and Imaging Initiative Grant 97793 | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | OLIGONUCLEOTIDES | - |
dc.subject | SEQUENCES | - |
dc.subject | PRIMERS | - |
dc.title | Site-specific fluorescent labeling of DNA using Staudinger ligation | - |
dc.type | Article | - |
dc.identifier.wosid | 000183071100027 | - |
dc.identifier.scopusid | 2-s2.0-0037570826 | - |
dc.type.rims | ART | - |
dc.citation.volume | 14 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 697 | - |
dc.citation.endingpage | 701 | - |
dc.citation.publicationname | BIOCONJUGATE CHEMISTRY | - |
dc.identifier.doi | 10.1021/bc0256392 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | SEO, TAE SEOK | - |
dc.contributor.nonIdAuthor | Wang, CCY | - |
dc.contributor.nonIdAuthor | Li, ZM | - |
dc.contributor.nonIdAuthor | Ruparel, H | - |
dc.contributor.nonIdAuthor | Ju, JY | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | OLIGONUCLEOTIDES | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordPlus | PRIMERS | - |
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