DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Joon Chul | ko |
dc.contributor.author | Kim, Do Yun | ko |
dc.contributor.author | Oh, Duk Jae | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.date.accessioned | 2013-03-06T15:05:20Z | - |
dc.date.available | 2013-03-06T15:05:20Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-07 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.13, no.4, pp.401 - 409 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/87353 | - |
dc.description.abstract | Recombinant CHO cells of DG44 origin (CS*13-1.00), expressing a chimeric antibody against the S surface antigen of the Hepatitis B virus, were cultivated in single-stage and two-stage depth filter perfusion systems (DFPS) under varying temperature, pH, and oxygen tension conditions to determine their effects on recombinant antibody production. A long-term culture was carried out in a single-stage depth filter for 81 days, during which an occasional clog interrupted the experiment. However, this problem was solved via trypsin injection. The DFPS showed a steady production of monoclonal antibody at a concentration of 100 similar to 150 mg/L. As the cultivation temperature was increased from 33 to 37 C, the monoclonal antibody (Mab) concentration increased from 80.33 to 133.47 mg/L. Likewise, the glucose uptake rate (GUR) and lactate production rate (LPR) also increased. With an increase in pH from 6.95 to 7.61, the Mab concentration increased from 61.64 to 94.31 mg/L. When the oxygen tension was increased from 60 to 80%, the Mab concentration increased from 93.78 to 128.30 mg/L. (c) KSBB. | - |
dc.language | English | - |
dc.publisher | Korean Soc Biotechnology & Bioengineering | - |
dc.subject | HAMSTER OVARY CELLS | - |
dc.subject | BACULOVIRUS EXPRESSION SYSTEM | - |
dc.subject | LAB-II CENTRIFUGE | - |
dc.subject | RCHO CELLS | - |
dc.subject | ERYTHROPOIETIN PRODUCTION | - |
dc.subject | RECYCLE BIOREACTOR | - |
dc.subject | CULTURE | - |
dc.subject | PROTEIN | - |
dc.subject | RETENTION | - |
dc.subject | QUALITY | - |
dc.title | Long-term operation of depth filter perfusion systems (DFPS) for monoclonal antibody production using recombinant CHO cells: Effect of temperature, pH, and dissolved oxygen | - |
dc.type | Article | - |
dc.identifier.wosid | 000258914500004 | - |
dc.identifier.scopusid | 2-s2.0-52749089488 | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 401 | - |
dc.citation.endingpage | 409 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.identifier.doi | 10.1007/s12257-008-0155-8 | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.nonIdAuthor | Oh, Duk Jae | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Chinese hamster ovary cells | - |
dc.subject.keywordAuthor | perfusion culture | - |
dc.subject.keywordAuthor | recombinant antibody | - |
dc.subject.keywordAuthor | two-stage depth fitter perfusion system | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | BACULOVIRUS EXPRESSION SYSTEM | - |
dc.subject.keywordPlus | LAB-II CENTRIFUGE | - |
dc.subject.keywordPlus | RCHO CELLS | - |
dc.subject.keywordPlus | ERYTHROPOIETIN PRODUCTION | - |
dc.subject.keywordPlus | RECYCLE BIOREACTOR | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | RETENTION | - |
dc.subject.keywordPlus | QUALITY | - |
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