DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sang Jick | ko |
dc.contributor.author | Jang, Myeong Hee | ko |
dc.contributor.author | Ahn, Hyun Joo | ko |
dc.contributor.author | Kim, Jin Hong | ko |
dc.contributor.author | Lim, Ji Hye | ko |
dc.contributor.author | Ryu, Chun Jeih | ko |
dc.contributor.author | Lin, Nam-Kyu | ko |
dc.contributor.author | Kim, Keun-Soo | ko |
dc.contributor.author | Park, Mi-Ju | ko |
dc.contributor.author | Park, Insoo | ko |
dc.contributor.author | Hong, Hyo Jeong | ko |
dc.date.accessioned | 2013-03-06T14:01:44Z | - |
dc.date.available | 2013-03-06T14:01:44Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-01 | - |
dc.identifier.citation | JOURNAL OF IMMUNOLOGICAL METHODS, v.329, no.1-2, pp.176 - 183 | - |
dc.identifier.issn | 0022-1759 | - |
dc.identifier.uri | http://hdl.handle.net/10203/87186 | - |
dc.description.abstract | In a previous study, we generated a murine hepatitis B virus (HBV)-neutralizing monoclonal antibody (mAb), KR127, that binds to an epitope (amino acids 37-45, NSNNPDWDF) of the preS1 antigen. Furthermore, an epitope tag, S1 (NANNPDWDF), was developed for protein tagging. The aim of the present study was to develop a high-affinity antibody to the same preS I epitope. Mice were immunized with the N-terminal domain of human thrombopoietin fused to the S I tag (nTPO-S1), and a phage-displayed chimeric Fab library was constructed and screened by panning against nTPO-S1. A high-affinity antibody (3-34) was selected that binds to the preS1 antigen. The IgG molecules of 3-34 showed approximately nine-fold higher affinity (K-D 1.2 nM) for preS1 compared with KR127 (K-D 10.4 nM), competed with KR127 for binding to the epitope, and bound to HBV particles. This study provides a simple and efficient way to develop a high-affinity antibody to a defined epitope by phage display of an immune antibody library. (C) 2007 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | HEPATITIS-B-VIRUS | - |
dc.subject | SINGLE-CHAIN FV | - |
dc.subject | RESPIRATORY SYNCYTIAL VIRUS | - |
dc.subject | MONOCLONAL-ANTIBODIES | - |
dc.subject | SURFACE-ANTIGEN | - |
dc.subject | HUMANIZED ANTIBODY | - |
dc.subject | BINDING-AFFINITY | - |
dc.subject | IN-VIVO | - |
dc.subject | PRES1 | - |
dc.subject | MATURATION | - |
dc.title | Selection of an affinity-matured antibody against a defined epitope by phage display of an immune antibody library | - |
dc.type | Article | - |
dc.identifier.wosid | 000253124100017 | - |
dc.identifier.scopusid | 2-s2.0-36849047754 | - |
dc.type.rims | ART | - |
dc.citation.volume | 329 | - |
dc.citation.issue | 1-2 | - |
dc.citation.beginningpage | 176 | - |
dc.citation.endingpage | 183 | - |
dc.citation.publicationname | JOURNAL OF IMMUNOLOGICAL METHODS | - |
dc.identifier.doi | 10.1016/j.jim.2007.10.009 | - |
dc.contributor.localauthor | Lin, Nam-Kyu | - |
dc.contributor.nonIdAuthor | Kim, Sang Jick | - |
dc.contributor.nonIdAuthor | Jang, Myeong Hee | - |
dc.contributor.nonIdAuthor | Ahn, Hyun Joo | - |
dc.contributor.nonIdAuthor | Kim, Jin Hong | - |
dc.contributor.nonIdAuthor | Lim, Ji Hye | - |
dc.contributor.nonIdAuthor | Ryu, Chun Jeih | - |
dc.contributor.nonIdAuthor | Kim, Keun-Soo | - |
dc.contributor.nonIdAuthor | Park, Mi-Ju | - |
dc.contributor.nonIdAuthor | Park, Insoo | - |
dc.contributor.nonIdAuthor | Hong, Hyo Jeong | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | monoclonal antibody | - |
dc.subject.keywordAuthor | phage display | - |
dc.subject.keywordAuthor | epitope tag | - |
dc.subject.keywordAuthor | hepatitis B virus preS1 | - |
dc.subject.keywordAuthor | affinity maturation | - |
dc.subject.keywordPlus | HEPATITIS-B-VIRUS | - |
dc.subject.keywordPlus | SINGLE-CHAIN FV | - |
dc.subject.keywordPlus | RESPIRATORY SYNCYTIAL VIRUS | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODIES | - |
dc.subject.keywordPlus | SURFACE-ANTIGEN | - |
dc.subject.keywordPlus | HUMANIZED ANTIBODY | - |
dc.subject.keywordPlus | BINDING-AFFINITY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | PRES1 | - |
dc.subject.keywordPlus | MATURATION | - |
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