DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiang, Min | ko |
dc.contributor.author | Shang, Longan | ko |
dc.contributor.author | Wei, Ping | ko |
dc.contributor.author | Yu, Ronghua | ko |
dc.contributor.author | Shen, Ning | ko |
dc.contributor.author | Ouyang, Pingkai | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.date.accessioned | 2013-03-06T13:57:26Z | - |
dc.date.available | 2013-03-06T13:57:26Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.citation | ENZYME AND MICROBIAL TECHNOLOGY, v.41, no.4, pp.407 - 412 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | http://hdl.handle.net/10203/87176 | - |
dc.description.abstract | In a 50L pilot scale reactor D-p-hydroxyphenylglycine (D-HPG) is produced enzymatically from DL-5-p-hydroxyphenylhydantoin (DL-HPH) with the resting cells of Burkholderia cepacia JS-02, requiring only corn steep liquor as a nitrogen source instead of the expensive yeast extract or peptone required by other strains. Both the fermentation process for preparing resting cells and the bioconversion were optimized in 5 L bench scale reactors. The cells showed the highest hydantoinase and carbarnoylase activities (0.640 and 0.304 U/mL-borth, respectively) at a fermentation of 18 h when Co2+ ions and DL-5-methylthioethyl hydantoin as an inducer were used. The optimal temperature and initial pH for bioconversion were 40 degrees C and 9, respectively. However, starting from the initial pH 9, pH dropped rapidly to near 7, at which level both key enzymes showed considerable activity. A pilot-scale bioconversion was carried out in a 50 L reactor with a productivity of 0.68 g/L h. Unlike conventional processes, this process using B. cepacia JS-02 can utilize inexpensive nitrogen and carbon sources for the production of the resting cells that contain the key enzymes. Also, it showed a high specific productivity during bioconversion without the use of a buffer solution. An economic analysis of this process showed that these advantages could lower production costs effectively. (c) 2007 Published by Elsevier Inc. | - |
dc.language | English | - |
dc.publisher | Elsevier Science Inc | - |
dc.subject | RECOMBINANT ESCHERICHIA-COLI | - |
dc.subject | AMINO-ACIDS | - |
dc.subject | D-HYDANTOINASE | - |
dc.subject | STRAINS | - |
dc.subject | CELLS | - |
dc.title | Pilot-scale production of D-p-hydroxyphenylglycine from DL-5-p-hydroxyphenylhydantoin by Burkholderia cepacia JS-02 | - |
dc.type | Article | - |
dc.identifier.wosid | 000248679900001 | - |
dc.identifier.scopusid | 2-s2.0-34447107312 | - |
dc.type.rims | ART | - |
dc.citation.volume | 41 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 407 | - |
dc.citation.endingpage | 412 | - |
dc.citation.publicationname | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.enzmictec.2007.02.010 | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.nonIdAuthor | Jiang, Min | - |
dc.contributor.nonIdAuthor | Shang, Longan | - |
dc.contributor.nonIdAuthor | Wei, Ping | - |
dc.contributor.nonIdAuthor | Yu, Ronghua | - |
dc.contributor.nonIdAuthor | Shen, Ning | - |
dc.contributor.nonIdAuthor | Ouyang, Pingkai | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | DL-5-p-Hydroxyphenylhydantoin | - |
dc.subject.keywordAuthor | D-carbamoylase | - |
dc.subject.keywordAuthor | D-hydantoinase | - |
dc.subject.keywordAuthor | D-p-hydroxyphenylglycine | - |
dc.subject.keywordAuthor | Burkholderia cepacia JS-02 | - |
dc.subject.keywordPlus | RECOMBINANT ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | D-HYDANTOINASE | - |
dc.subject.keywordPlus | STRAINS | - |
dc.subject.keywordPlus | CELLS | - |
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