Two-Stage Depth Filter Perfusion Culture for Recombinant Antibody Production by Recombinant Chinese Hamster Ovary Cell

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A rCHO cell line of DUKX origin 26*-320, producing recombinant antibody against the human platelet, was cultivated in a two-stage depth filter perfusion system (DFPS) for 20 days in order to attain high recombinant antibody concentration. The productivity of the first stage DFPS bioreactor reached 53 times that of the batch culture in a controlled stirred tank reactor and was showed 12.1 mg/L antibody concentration at a perfusion rate of 6.0 d(-1). Glucose concentration in the first DFPS was maintained at 1.5 g/L to avoid cell damage in the perfusion culture. A second stage DFPS system was attached to the first DFPS, which resulted in a low glucose concentration of 0.02 g/L and a high antibody concentration of 23.9 mg/L. The two-stage depth filter perfusion culture yielded 60% higher product concentration than the batch and 49-fold higher productivity of 69.3 mg/L/d in comparison with that (1.4 mg/L/d) in a batch system. Furthermore, antibody concentration of the second stage was 97% higher than that of the first stage, and the antibody productivities were comparable to that of the first stage. This two-stage DFPS system also showed potential for higher titer production of recombinant antibody and high volumetric productivity for long-term culture of bio-pharmaceutical substances. (C) KSBB
Publisher
Korean Soc Biotechnology & Bioengineering
Issue Date
2008-09
Language
English
Article Type
Article
Keywords

LAB-II CENTRIFUGE; RCHO CELLS; RECYCLE BIOREACTOR; MAMMALIAN-CELLS; CHO CELLS; SYSTEM; TEMPERATURE; ERYTHROPOIETIN; STIMULATION

Citation

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.13, no.5, pp.560 - 565

ISSN
1226-8372
DOI
10.1007/s12257-008-0003-x
URI
http://hdl.handle.net/10203/87159
Appears in Collection
CBE-Journal Papers(저널논문)
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