Cohnella panacarvi sp nov., a xylanolytic bacterium isolated from ginseng cultivating soil

Abstract

A Gram-positive, aerobic, rod-shaped, nonmotile, endospore-forming bacterium, designated Gsoil 349(T), was isolated from soil of a ginseng field and characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene sequences revealed that the strain Gsoil 349(T) belongs to the family Paenibacillaceae, and the sequence showed closest similarity with Cohnella thermotolerans DSM 17683(T) (94.1%) (93.6%). The and Cohnella hongkongensis DSM 17642 strain showed less than 91.3% 16S rRNA gene sequence similarity with Poenibacillus species. In addition, the presence of MK-7 as the major menaquinone and anteiso-C-15:0, iso-C-16:0, and C-16:0 as major fatty acids suggested its affiliation to the genus Cohnello. The G+C content of the genomic DNA was 53.4 mol%. On the basis of its phenotypic characteristics and phylogenetic distinctiveness, strain Gsoil 349(T) should be treated as a novel species within the genus Cohnella for which the name Cohnella panocarvi sp. nov. is proposed. The type strain is Gsoil 349(T) (=KCTC 13060(T) = DSM 18696(T)).