DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Kyoung G | ko |
dc.contributor.author | Pillai, Shreekumar R. | ko |
dc.contributor.author | Singh, Shree R. | ko |
dc.contributor.author | Willing, Gerold A. | ko |
dc.date.accessioned | 2013-03-06T10:06:31Z | - |
dc.date.available | 2013-03-06T10:06:31Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-03 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.99, no.4, pp.949 - 959 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/86642 | - |
dc.description.abstract | The investigation of Protein A and antibody adsorption on surfaces in a biological environment is an important and fundamental step for increasing biosensor sensitivity and specificity. The atomic force microscope (AFM) is a powerful tool that is frequently used to characterize surfaces coated with a variety of molecules. We used AFM in conjunction with scanning electron microscopy to characterize the attachment of protein A and its subsequent binding to the antibody and Salmonella bacteria using a gold quartz crystal. The rms roughness of the base gold surface was determined to be approximately 1.30 nm. The average step height change between the solid gold and protein A layer was approximately 3.0 +/- 1.0 nm, while the average step height of the protein A with attached antibody was approximately 6.0 +/- 1.0 nm. We found that the antibodies did not completely cover the protein A layer, instead the attachment follows an island model. Salt crystals and water trapped under the protein A layer were also observed. The uneven adsorption of antibodies onto the biosensor surface might have led to a decrease in the sensitivity of the biosensor. The presence of salt crystals and water under the protein A layer may deteriorate the sensor specificity. in this report, we have discussed the application and characterization of protein A bound to antibodies which can be used to detect bacterial and viral pathogens. | - |
dc.language | English | - |
dc.publisher | JOHN WILEY & SONS INC | - |
dc.subject | QUARTZ-CRYSTAL MICROBALANCE | - |
dc.subject | PIEZOELECTRIC BIOSENSOR | - |
dc.subject | SPRING CONSTANT | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | GOLD | - |
dc.subject | FABRICATION | - |
dc.title | The investigation of protein a and Salmonella antibody adsorption onto biosensor surfaces by atomic force microscopy | - |
dc.type | Article | - |
dc.identifier.wosid | 000253277300023 | - |
dc.identifier.scopusid | 2-s2.0-39549109106 | - |
dc.type.rims | ART | - |
dc.citation.volume | 99 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 949 | - |
dc.citation.endingpage | 959 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.21644 | - |
dc.contributor.localauthor | Lee, Kyoung G | - |
dc.contributor.nonIdAuthor | Pillai, Shreekumar R. | - |
dc.contributor.nonIdAuthor | Singh, Shree R. | - |
dc.contributor.nonIdAuthor | Willing, Gerold A. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | atomic force microscopy | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordAuthor | protein A | - |
dc.subject.keywordAuthor | quartz crystal | - |
dc.subject.keywordAuthor | scanning electron microscopy | - |
dc.subject.keywordPlus | QUARTZ-CRYSTAL MICROBALANCE | - |
dc.subject.keywordPlus | PIEZOELECTRIC BIOSENSOR | - |
dc.subject.keywordPlus | SPRING CONSTANT | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | FABRICATION | - |
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