A new microbiosensor has been proposed and fabricated for the measurement of glucose concentration in whole blood, The proposed microbiosensor has been monolithically integrated with enzymatic metal microelectrode array in a micromachined chamber attached to a microsyringe. The fabricated microbiosensor has a high sensitivity of 470 nA/cm(2) mM for detecting glucose levels in a wide linear range of 0-50 mM in potassium phosphate buffer solution (pH 7.0). (C) 2002 Elsevier Science B.V. All rights reserved.