For applications such as sequencing, transfection, and in vitro transcription. IVR products have to be subcloned into plasmids. Many strategies are used for cloning, blunt-end ligation or the incorporation of restriction endonuclease sites into PCR primers for appropriate vectors. However, the most convenient and direct method is T/A cloning. In this study, we developed two of the pGEM-7Zf(+) phagemid T-tail vectors using AhaI-restriction endonuclease sites. and these T vectors have all the features of pGFM-7Zf(+): f1 ori, T7, and SP6 RNA polymerase promoters. the alpha-peptide coding region of beta-galactosidase for X-gal blue/white color selection. the beta-lactamase gene for recombinant colony selection, and binding sites for pUC/M13 forward and reverse sequencing primers. These AhdI-containing phagemid vectors, pGEM-NJ105 and pGEM-NJI07. are useful Cot, the easy and inexpensive preparation of T vectors and direct cloning of PCR products. (C) 2002 Elsevier Science (USA). All rights reserved.