DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, BH | ko |
dc.contributor.author | Seo, DJ | ko |
dc.contributor.author | Nam, SW | ko |
dc.contributor.author | Na, JG | ko |
dc.contributor.author | Chang, YongKeun | ko |
dc.date.accessioned | 2013-03-03T04:54:24Z | - |
dc.date.available | 2013-03-03T04:54:24Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1997-08 | - |
dc.identifier.citation | JOURNAL OF FERMENTATION AND BIOENGINEERING, v.84, no.5, pp.466 - 470 | - |
dc.identifier.issn | 0922-338X | - |
dc.identifier.uri | http://hdl.handle.net/10203/77313 | - |
dc.description.abstract | Fed-batch fermentation was conducted to overproduce human lipocortin-1 (LC1) in recombinant Saccharomyces cerevisiae controlled by the GAL10 promoter. To optimize the feeding strategy, various fed-batch culture modes were performed with concentrated feed media containing carbon mixtures of galactose (Gal) and glucose (Glu) at three different concentration ratios (Gal:Glu ratios): 9:1, 1:1, 1:9. The cell growth, expression level of LC1, and the plasmid stability were investigated under these fed-batch culture modes, While both glucose and galactose were being fed to the fermenter, the glucose concentration in the culture broth was kept below 1 g/l for efficient gene expression. High cell concentrations of greater than 100 g dry cell weight/l mere achieved with these fed-batch culture modes. A significant amount of intact LC1 was found to be secreted into the culture medium, but proteolytically cleaved products (des1-26-LC1) were also observed in the culture medium. The fed-batch fermentation with the feed medium at a Gal: Glu ratio of 1:1 resulted in the highest LC1 total (intact LC1+des1-26-LC1) concentration of 500 mg/l, which corresponded to 1.73- and 1.83-fold increases over that produced with the media at Gal: Glu ratios of 1:9 and 9:1, respectively. | - |
dc.language | English | - |
dc.publisher | SOC FERMENTATION BIOENGINEERING | - |
dc.subject | GROWTH-RATE | - |
dc.subject | EXPRESSION | - |
dc.subject | YEAST | - |
dc.subject | GENE | - |
dc.subject | PROTEINS | - |
dc.subject | STABILITY | - |
dc.subject | UPSTREAM | - |
dc.subject | CULTURE | - |
dc.title | Optimization of feeding strategy for overproduction of human lipocortin-I in Saccharomyces cerevisiae controlled by the GAL10 promoter | - |
dc.type | Article | - |
dc.identifier.wosid | 000071769400013 | - |
dc.identifier.scopusid | 2-s2.0-0031337578 | - |
dc.type.rims | ART | - |
dc.citation.volume | 84 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 466 | - |
dc.citation.endingpage | 470 | - |
dc.citation.publicationname | JOURNAL OF FERMENTATION AND BIOENGINEERING | - |
dc.contributor.localauthor | Chang, YongKeun | - |
dc.contributor.nonIdAuthor | Chung, BH | - |
dc.contributor.nonIdAuthor | Seo, DJ | - |
dc.contributor.nonIdAuthor | Nam, SW | - |
dc.contributor.nonIdAuthor | Na, JG | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | human lipocortin-I | - |
dc.subject.keywordAuthor | fed-batch | - |
dc.subject.keywordPlus | GROWTH-RATE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | UPSTREAM | - |
dc.subject.keywordPlus | CULTURE | - |
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