Mass production of poly(3-hydroxybutyrate) by fed-batch cultures of Ralstonia eutropha with nitrogen and phosphate limitation

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dc.contributor.authorRyu, HWko
dc.contributor.authorCho, KSko
dc.contributor.authorKim, BSko
dc.contributor.authorChang, YongKeunko
dc.contributor.authorChang, Ho Namko
dc.contributor.authorShim, HJko
dc.date.accessioned2013-03-03T04:41:15Z-
dc.date.available2013-03-03T04:41:15Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1999-12-
dc.identifier.citationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.9, no.6, pp.751 - 756-
dc.identifier.issn1017-7825-
dc.identifier.urihttp://hdl.handle.net/10203/77268-
dc.description.abstractFor mass production of poly(3-hydroxybutyrate) (PHB), high cell density cultures of Ralstonia eutropka were carried out in 2.5-1 and 60-1 fermenters by two fed-batch culture techniques of nitrogen and phosphate limitation. When the nitrogen limitation technique was employed using both an on-line glucose monitoring and control system, a high concentration level of PHB (121 g/l) was obtained in the small-scale fermenter of 2.5 1. However, the PHB concentration obtained in a large-scale fermenter of 60 1 only turned out to be 60 g/l. In contrast, when another fed-batch culture technique of the phosphate-limitation employing dissolved oxygen (DO) stat glucose feeding was used, a large amount of PHB was successfully produced in both 60-1 and 2.5-1 fermenters. In a 2.5-1 fermenter, concentrations of PHB and cells obtained in 58 h were 175 and 210 g/l, respectively, which corresponded to the PHB productivity level of 3.02 g/l/h. In a 60-1 fermenter, a final cell concentration of 221 g/l and a PHB concentration of 180 g/l with PHB productivity level of 3.75 g/l/h were obtained in 48 h. PHB content and yield from glucose were 81% and 0.38 g PHB/g glucose, respectively. These data suggest that the phosphate limitation technique is more effective compared to nitrogen limitation in the mass production of PHB by R. eutropha of a large scale.-
dc.languageEnglish-
dc.publisherKOREAN SOC MICROBIOLOGY BIOTECHNOLOGY-
dc.subjectPOLY-BETA-HYDROXYBUTYRATE-
dc.subjectRECOMBINANT ESCHERICHIA-COLI-
dc.subjectAZOTOBACTER-VINELANDII UWD-
dc.subjectALCALIGENES-EUTROPHUS-
dc.subjectMETHYLOBACTERIUM-ORGANOPHILUM-
dc.subjectDENSITY CULTIVATION-
dc.subjectPENTANOIC ACIDS-
dc.subjectPOLYHYDROXYALKANOATES-
dc.subjectBIOSYNTHESIS-
dc.subjectPHB-
dc.titleMass production of poly(3-hydroxybutyrate) by fed-batch cultures of Ralstonia eutropha with nitrogen and phosphate limitation-
dc.typeArticle-
dc.identifier.wosid000084577500010-
dc.identifier.scopusid2-s2.0-6744266675-
dc.type.rimsART-
dc.citation.volume9-
dc.citation.issue6-
dc.citation.beginningpage751-
dc.citation.endingpage756-
dc.citation.publicationnameJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.contributor.localauthorChang, YongKeun-
dc.contributor.localauthorChang, Ho Nam-
dc.contributor.nonIdAuthorRyu, HW-
dc.contributor.nonIdAuthorCho, KS-
dc.contributor.nonIdAuthorKim, BS-
dc.contributor.nonIdAuthorShim, HJ-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorpoly(3-hydroxybutyrate)-
dc.subject.keywordAuthorRalstonia eutropha-
dc.subject.keywordAuthorfed-batch culture-
dc.subject.keywordAuthorphosphate limitation-
dc.subject.keywordPlusPOLY-BETA-HYDROXYBUTYRATE-
dc.subject.keywordPlusRECOMBINANT ESCHERICHIA-COLI-
dc.subject.keywordPlusAZOTOBACTER-VINELANDII UWD-
dc.subject.keywordPlusALCALIGENES-EUTROPHUS-
dc.subject.keywordPlusMETHYLOBACTERIUM-ORGANOPHILUM-
dc.subject.keywordPlusDENSITY CULTIVATION-
dc.subject.keywordPlusPENTANOIC ACIDS-
dc.subject.keywordPlusPOLYHYDROXYALKANOATES-
dc.subject.keywordPlusBIOSYNTHESIS-
dc.subject.keywordPlusPHB-
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