DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.date.accessioned | 2013-03-02T18:59:20Z | - |
dc.date.available | 2013-03-02T18:59:20Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995-01 | - |
dc.identifier.citation | CANADIAN JOURNAL OF MICROBIOLOGY, v.41, pp.207 - 215 | - |
dc.identifier.issn | 0008-4166 | - |
dc.identifier.uri | http://hdl.handle.net/10203/75035 | - |
dc.description.abstract | A number of Escherichia coli strains including K12, B, W, XL1-Blue, DH5 alpha, HB101, JM109, and C600 were transformed with the stable high-copy-number plasmid pSYL105 containing the Alcaligenes eutrophus polyhydroxyalkanoic acid biosynthesis genes, and were subsequently compared for their ability to synthesize and accumulate poly(3-hydroxybutyric acid) (PHB). The rate of PHB synthesis, the extent of PHB accumulation, and PHB yield from glucose varied considerably from one strain to another. Strains XL1-Blue and B harboring pSYL105 synthesized PHB at the highest rate to a final concentration of ca. 7 g/L in complex medium containing 20 g glucose/L. With an aim to reduce the cost of the medium, the effect on PHB accumulation of supplementing a defined medium with complex nitrogen sources was examined. A PHB concentration of 81 g/L could be obtained in 41 h from a pH-stat fed-batch culture of XL1-Blue(pSYL105) in a semidefined medium. When the availability of acetyl-CoA was increased by supplementing the medium with complex nitrogen sources, amino acids, or oleic acid, PHB synthesis by recombinant E. coli was enhanced. | - |
dc.language | English | - |
dc.publisher | CANADIAN SCIENCE PUBLISHING | - |
dc.title | Production of poly(3-hydroxybutyric acid) by recombinant Escherichia coli strains: genetic and fermentation studies | - |
dc.type | Article | - |
dc.identifier.wosid | A1995RE28400029 | - |
dc.identifier.scopusid | 2-s2.0-0029125406 | - |
dc.type.rims | ART | - |
dc.citation.volume | 41 | - |
dc.citation.beginningpage | 207 | - |
dc.citation.endingpage | 215 | - |
dc.citation.publicationname | CANADIAN JOURNAL OF MICROBIOLOGY | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordAuthor | POLYHYDROXYALKANOIC ACID | - |
dc.subject.keywordAuthor | POLY(3-HYDROXYBUTYRIC ACID) | - |
dc.subject.keywordAuthor | FED BATCH | - |
dc.subject.keywordAuthor | ESCHERICHIA COLI | - |
dc.subject.keywordPlus | POLY-BETA-HYDROXYBUTYRATE | - |
dc.subject.keywordPlus | ALCALIGENES-EUTROPHUS H16 | - |
dc.subject.keywordPlus | CELL-DENSITY CULTIVATION | - |
dc.subject.keywordPlus | FED-BATCH CULTURE | - |
dc.subject.keywordPlus | PHB | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | SUCROSE | - |
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