DC Field | Value | Language |
---|---|---|
dc.contributor.author | 류희욱 | ko |
dc.contributor.author | 조경숙 | ko |
dc.contributor.author | 장용근 | ko |
dc.date.accessioned | 2013-03-02T18:12:06Z | - |
dc.date.available | 2013-03-02T18:12:06Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-01 | - |
dc.identifier.citation | KSBB JOURNAL, v.13, no.2, pp.187 - 194 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74848 | - |
dc.description.abstract | For mass production of polyhydroxyalkanoates (PHA), high cell density cultures of Alcaligenes eutrophus by fed-batch culture under phosphate-limitation condition has been investigated. PHA accumulation by the regulation by the regulation of initial phosphate concentration could be automatically induced, and high density cell culture above 200 g/L also could be successfully produced. The production of Poly-β-hydroxybutyrate (PHB) and dry cell weight increased with increasing the initial phosphate concentration. When the initial concentrations of phosphate were in the ranges of 1.5~4.5 g-PO4/L, PHB and dry cell weight obtained were 83~266 g/L and 61~216 g/L, respectively, and PHB productivity was in the ranges of 1.35~3.10 g/L.h. When a mixture of glucose and propionic acid is used as carbon sources, poly(3-hydroxybutyrate-co-poly-3-hydroxyvalerate), P(3HB-co-3HV), could be also successfully produced under phosphate limitation condition. When the mole ratio of propionic acid to glucose in the feeding solution is 0.22, a final dry cell weight of 150 g/L and a P(3-HB-co-3HV) of 90 g/L were produced. Morphological changes and size distribution of PHB granules synthesized in A. eutrophus under phosphate-limitation condition are determined by TEM during the course of fed-batch. Mean granule diameters of PHB produced are in the range of 0.36~0.39 μm, and mean cell size was elongated from 0.54~0.59 μmx 1.3~1.5 μm to 0.83~0.89 μm x2.0~2.3 μm. Phosphate concentration in media did not affect size distribution of PHB granule and cell. | - |
dc.language | Korean | - |
dc.publisher | 한국생물공학회 | - |
dc.title | 인산염 제한하에서 Alcaligenes eutrophus의 유가식 배양에 의한 Polyhydroxyalkanoates의 대량생산과 축적특성 | - |
dc.title.alternative | Mass Production and Accumulation Characteristics of Polyhydroxyalkanoates by Fed-batch culture of Alcaligenes eutrophus under Phosphate Limitation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 187 | - |
dc.citation.endingpage | 194 | - |
dc.citation.publicationname | KSBB JOURNAL | - |
dc.contributor.localauthor | 장용근 | - |
dc.contributor.nonIdAuthor | 류희욱 | - |
dc.contributor.nonIdAuthor | 조경숙 | - |
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