Continuous production of galacto-oligosaccharides from lactose by Bullera singularis beta-galactosidase immobilized in chitosan beads

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Galacto-oligosaccharides (GalOS) were continuously produced using lactose and immobilized beta-galactosidase from Bullera singularis ATCC 24193 in a packed bed reactor. Partially purified beta-galactosidase was immobilized in Chitopearl BCW 3510 bead (970 GU/g resin) by simple adsorption. 55% (w/w) oligosaccharides was obtained continuously with a productivity of 4.4 g/(litre-h) from 100 g/litre lactose solution during a 15-day operation. Batch productivity was 6.5 g GalOS/(litre-h) from 300 g/litre lactose. (C) 1998 Elsevier Science Ltd. All rights reserved.
Publisher
ELSEVIER SCI LTD
Issue Date
1998-11
Language
English
Article Type
Article
Keywords

BACILLUS-CIRCULANS; PURIFICATION

Citation

PROCESS BIOCHEMISTRY, v.33, no.8, pp.787 - 792

ISSN
0032-9592
URI
http://hdl.handle.net/10203/70282
Appears in Collection
CBE-Journal Papers(저널논문)
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