Continuous production of galacto-oligosaccharides from lactose by Bullera singularis beta-galactosidase immobilized in chitosan beads
Galacto-oligosaccharides (GalOS) were continuously produced using lactose and immobilized beta-galactosidase from Bullera singularis ATCC 24193 in a packed bed reactor. Partially purified beta-galactosidase was immobilized in Chitopearl BCW 3510 bead (970 GU/g resin) by simple adsorption. 55% (w/w) oligosaccharides was obtained continuously with a productivity of 4.4 g/(litre-h) from 100 g/litre lactose solution during a 15-day operation. Batch productivity was 6.5 g GalOS/(litre-h) from 300 g/litre lactose. (C) 1998 Elsevier Science Ltd. All rights reserved.
- Publisher
- ELSEVIER SCI LTD
- Issue Date
- 1998-11
- Language
- English
- Article Type
- Article
- Keywords
BACILLUS-CIRCULANS; PURIFICATION
- Citation
PROCESS BIOCHEMISTRY, v.33, no.8, pp.787 - 792
- ISSN
- 0032-9592
- Appears in Collection
- CBE-Journal Papers(저널논문)
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