BIODEGRADATION OF CYANIDE COMPOUNDS BY PSEUDOMONAS-FLUORESCENS IMMOBILIZED ON ZEOLITE

Cited 29 time in webofscience Cited 0 time in scopus
  • Hit : 291
  • Download : 0
A cyanide-degrading bacteria, Pseudomonas fluorescens NCIB 11764, was used for the treatment of wastewater containing cyanide. After successive subculture for 2 months in cyanide-containing medium, cultures were fully adapted to cyanide and able to grow in a medium containing up to 260 mg l(-1) of cyanide. By fed-batch culture supplying only cyanide, it was confirmed that cyanide was the limiting nutrient as a nitrogen source. Ammonia was found to inhibit cyanide degradation, and the rate of degradation was halved when the ammonia concentration was higher than 1 mM. In a 15-l jar fermenter, 26 mg l(-1) of cyanide was consumed almost completely within 48 h and the final yield of growth on cyanide was calculated as 4.21 mg dry cell wt mg(-1) CN. Cells of P. fluorescens were immobilized on zeolite and applied to a modified activated sludge-type reactor to mimic the actual activated sludge system of a coke-plant wastewater treatment system. In this immobilized system operated batchwise, 26 mg l(-1) of cyanide was degraded completely by 8 g l(-1) of immobilized particles within 10 h. The same reactor system was operated in a continuous mode, and the cyanide concentration of the outlet reached the stationary-state level of less than 0.1 mg l(-1) within 2 days at 0.041 h(-1) of dilution rate and within 3 days at 0.082 h(-1) of dilution rate, respectively.
Publisher
BUTTERWORTH-HEINEMANN
Issue Date
1994-06
Language
English
Article Type
Article
Keywords

UTILIZES CYANIDE; NITROGEN; GROWTH; STRAIN

Citation

ENZYME AND MICROBIAL TECHNOLOGY, v.16, no.6, pp.529 - 533

ISSN
0141-0229
URI
http://hdl.handle.net/10203/66840
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 29 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0