GALACTO-OLIGOSACCHARIDE PRODUCTION BY BETA-GALACTOSIDASE IN HYDROPHOBIC ORGANIC MEDIA

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Production of galacto-oligosaccharide (GO), including trisaccharide and tetrasaccharide, was performed using a beta-galactosidase in water-hydrophobic solvent mixtures. A maximum GO concentration of 45% (w/w) was attained in a 95% cyclohexane / 5% water mixture from a 55% (w/w) of lactose at 60 degrees C and pH 6.0, while a maximum of 38% GO in aqueous media. GO production decreased with an increase in surfactant concentration. The optimum water content for GO production showed a broad range from 2.5 to 10% (v/v). Solvent properties, such as log P and the dipole moment, had no relation to GO production.
Publisher
CHAPMAN HALL LTD
Issue Date
1994-11
Language
English
Article Type
Article
Keywords

SOLVENTS; GLYCOSIDASES; GLUCOAMYLASE

Citation

BIOTECHNOLOGY LETTERS, v.16, no.11, pp.1157 - 1162

ISSN
0141-5492
URI
http://hdl.handle.net/10203/66837
Appears in Collection
CBE-Journal Papers(저널논문)
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