Effects of mechanical environment on regulation of structure and function in vascular endothelial cells = 기계적 환경에 의한 혈관 내피세포의 구조와 기능 변화에 대한 연구

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Vascular endothelial cells (VEC) are continuously exposed to hemodynamic forces of tensile stress due to blood vessel dilations, and shear stress and pressure due to blood flow. These hemodynamic forces play an important role in VEC physiology and are closely related to the cardiovascular diseases. One such example is the atherosclerosis caused by an unusually low shear stress due to abnormal flow patterns in the blood vessel. Alterations in the shear stress caused by non-physiological flow conditions induce various changes in the structure and function of VEC by regulating the cytoskeleton and intercellular junction proteins. Therefore it is important to investigate the correlation between these protein expressions and the various flow conditions. We first develop a parallel plate flow chamber designed based on 3-D CFD (computational fluid dynamics) technique. In cultured VEC, the effects of various shear stresses and flow chamber geometries on junction and cytoskeleton proteins are observed using immunostaining and conventional microscopy. Experimental results show that cells have tendency of migrating against the flow and orienting more parallel to the direction of flow in a higher stress than a lower stress as time progresses. Moreover, cells divide less frequently as the magnitude of shear stress increases. In addition, the longer and thicker actin stress fibers form across the cytoplasm and connexin43 shows up-regulated expression as the duration of stress increases. In a set of experiments with hemodynamic features of atherosclerotic lesion-prone region, the cells near zero shear stress regions round up and become abnormally aggregated. Our findings provide new insights into the role of the mechanical stress on cytoskeleton rearrangement and the junction protein expression in regions prone to the pathogenesis of cardiovascular diseases such as atherosclerosis.
Advisors
신현정researcherShin, Jennifer Hyun-Jongresearcher
Description
한국과학기술원 : 기계공학전공,
Publisher
한국과학기술원
Issue Date
2007
Identifier
268783/325007  / 020053880
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 기계공학전공, 2007. 8, [ ix, 78 p. ]

Keywords

vascular endothelial cell; fluid shear stress; cytoskeleton; junction protein; atherosclerosis; 혈관내피세포; 전단응력; 세포내골격; 세포결합단백질; 죽상동맥경화; vascular endothelial cell; fluid shear stress; cytoskeleton; junction protein; atherosclerosis; 혈관내피세포; 전단응력; 세포내골격; 세포결합단백질; 죽상동맥경화

URI
http://hdl.handle.net/10203/45584
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=268783&flag=dissertation
Appears in Collection
ME-Theses_Master(석사논문)
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