DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hansol | ko |
dc.contributor.author | Lee, Seoyoung | ko |
dc.contributor.author | Ju, Yong | ko |
dc.contributor.author | Kim, Hyoyong | ko |
dc.contributor.author | Jang, Hyowon | ko |
dc.contributor.author | Park, Yeonkyung | ko |
dc.contributor.author | Lee, Sang Mo | ko |
dc.contributor.author | Yong, Dongeun | ko |
dc.contributor.author | Kang, Taejoon | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2024-06-25T12:00:08Z | - |
dc.date.available | 2024-06-25T12:00:08Z | - |
dc.date.created | 2024-06-25 | - |
dc.date.created | 2024-06-25 | - |
dc.date.issued | 2024-06 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.253 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/320011 | - |
dc.description.abstract | We herein present a multifunctional self-priming hairpin probe-based isothermal amplification, termed MSH, enabling one-pot detection of target nucleic acids. The sophisticatedly designed multifunctional self-priming hairpin (MSH) probe recognizes the target and rearranges to prime itself, triggering the amplification reaction powered by the continuously repeated extension, nicking, and target recycling. As a consequence, a large number of double-stranded DNA (dsDNA) amplicons are produced that could be monitored in real-time using a dsDNAintercalating dye. Based on this unique design approach, the nucleocapsid (N) and the open reading frame 1 ab (ORF1ab) genes of SARS-CoV-2 were successfully detected down to 1.664 fM and 0.770 fM, respectively. The practical applicability of our method was validated by accurately diagnosing 60 clinical samples with 93.33% sensitivity and 96.67% specificity. This isothermal one-pot MSH technique holds great promise as a point-of-care testing protocol for the reliable detection of a wide spectrum of pathogens, particularly in resource-limited settings. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Multifunctional self-priming hairpin probe-based isothermal nucleic acid amplification and its applications for COVID-19 diagnosis | - |
dc.type | Article | - |
dc.identifier.wosid | 001205793700001 | - |
dc.identifier.scopusid | 2-s2.0-85186761659 | - |
dc.type.rims | ART | - |
dc.citation.volume | 253 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2024.116147 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Yong, Dongeun | - |
dc.contributor.nonIdAuthor | Kang, Taejoon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Self -priming hairpin probe | - |
dc.subject.keywordAuthor | Isothermal amplification | - |
dc.subject.keywordAuthor | Molecular diagnostics | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | COVID-19 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.