DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Young Hun | ko |
dc.contributor.author | Jeon, Nayeong | ko |
dc.contributor.author | Park, Sujin | ko |
dc.contributor.author | Choi, Siyoung Q. | ko |
dc.contributor.author | Lee, Eunji | ko |
dc.contributor.author | Li, Sheng | ko |
dc.date.accessioned | 2024-06-19T14:00:20Z | - |
dc.date.available | 2024-06-19T14:00:20Z | - |
dc.date.created | 2024-06-19 | - |
dc.date.created | 2024-06-19 | - |
dc.date.created | 2024-06-19 | - |
dc.date.issued | 2024-04 | - |
dc.identifier.citation | ACS Macro Letters, v.13, no.5, pp.528 - 536 | - |
dc.identifier.issn | 2161-1653 | - |
dc.identifier.uri | http://hdl.handle.net/10203/319878 | - |
dc.description.abstract | We report the complexation of poly(ethylene glycol) conjugated double-stranded oligoDNA (PEG-(ds)oligoDNA) with imidazolium-based ionic liquids (ILs) to form polyelectrolyte complex aggregates (PCAs). The PEG-(ds)oligoDNA conjugates are prepared following a solution-phase coupling reaction. The binding of PEG-(ds)oligoDNA with either 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]) or 1-hexyl-3-methylimidazolium tetrafluoroborate ([HMIM][BF4]) is confirmed by a fluorescence displacement assay. Both ILs show stronger binding affinity to PEG-(ds)oligoDNA than bare (ds)oligoDNA due to the PEG-assisted increase in IL cation concentration in the vicinity of (ds)oligoDNA. The complex morphology formed at various amine (N) to phosphate (P) ratios is also examined. At high N/P ratios above 4, nanosized PCAs are formed, driven by a counterion-mediated attraction among the IL-bound (ds)oligoDNA segments and stabilized by the conjugated PEG segments. The PCAs exhibit near-neutral surface charges and resistance to DNase degradation, suggesting their potential use in gene delivery applications. | - |
dc.language | English | - |
dc.publisher | American Chemical Society (ACS) | - |
dc.title | Complexation of Poly(ethylene glycol)-(ds)OligoDNA Conjugates with Ionic Liquids | - |
dc.type | Article | - |
dc.identifier.wosid | 001204941300001 | - |
dc.identifier.scopusid | 2-s2.0-85190741043 | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 528 | - |
dc.citation.endingpage | 536 | - |
dc.citation.publicationname | ACS Macro Letters | - |
dc.identifier.doi | 10.1021/acsmacrolett.4c00028 | - |
dc.contributor.localauthor | Choi, Siyoung Q. | - |
dc.contributor.localauthor | Li, Sheng | - |
dc.contributor.nonIdAuthor | Jeon, Nayeong | - |
dc.contributor.nonIdAuthor | Lee, Eunji | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ANTISENSE OLIGONUCLEOTIDE | - |
dc.subject.keywordPlus | DELIVERY-SYSTEM | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | PRECIPITATION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | COUNTERIONS | - |
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