DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Siseon | ko |
dc.contributor.author | Kang, Minsik | ko |
dc.contributor.author | Jung, Chan-Duck | ko |
dc.contributor.author | Bae, Jung-Hoon | ko |
dc.contributor.author | Lee, Ju Young | ko |
dc.contributor.author | Park, Young-Kwon | ko |
dc.contributor.author | Joo, Jeong Chan | ko |
dc.contributor.author | Kim, Hoyong | ko |
dc.contributor.author | Sohn, Jung-Hoon | ko |
dc.contributor.author | Sung, Bong Hyun | ko |
dc.date.accessioned | 2023-10-30T07:00:19Z | - |
dc.date.available | 2023-10-30T07:00:19Z | - |
dc.date.created | 2023-10-30 | - |
dc.date.created | 2023-10-30 | - |
dc.date.created | 2023-10-30 | - |
dc.date.issued | 2023-11 | - |
dc.identifier.citation | BIORESOURCE TECHNOLOGY, v.388 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | http://hdl.handle.net/10203/313911 | - |
dc.description.abstract | Pseudomonas putida is a promising strain for lignin valorisation. However, there is a dearth of stable and efficient systems for secreting enzymes to enhance the process. Therefore, a novel secretion system for recombinant lignin-depolymerising peroxidase was developed. By adopting a flagellar type III secretion system, P. putida KT -M2, a secretory host strain, was constructed and an optimal secretion signal fusion partner was identified. Application of the dye-decolourising peroxidase of P. putida to this system resulted in efficient oxidation activity of the cell-free supernatant against various chemicals, including lignin model compounds. This peroxidase-secreting strain was examined to confirm its lignin utilisation capability, resulting in the efficient assimilation of various lignin substrates with 2.6-fold higher growth than that of the wild-type strain after 72 h of cultivation. Finally, this novel system will lead efficient bacterial lignin breakdown and utilization through enzyme secretion, paving the way for sustainable lignin-consolidated bioprocessing. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Development of novel recombinant peroxidase secretion system from Pseudomonas putida for lignin valorisation | - |
dc.type | Article | - |
dc.identifier.wosid | 001083766400001 | - |
dc.identifier.scopusid | 2-s2.0-85171805049 | - |
dc.type.rims | ART | - |
dc.citation.volume | 388 | - |
dc.citation.publicationname | BIORESOURCE TECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.biortech.2023.129779 | - |
dc.contributor.localauthor | Lee, Ju Young | - |
dc.contributor.nonIdAuthor | Lee, Siseon | - |
dc.contributor.nonIdAuthor | Kang, Minsik | - |
dc.contributor.nonIdAuthor | Jung, Chan-Duck | - |
dc.contributor.nonIdAuthor | Bae, Jung-Hoon | - |
dc.contributor.nonIdAuthor | Park, Young-Kwon | - |
dc.contributor.nonIdAuthor | Joo, Jeong Chan | - |
dc.contributor.nonIdAuthor | Kim, Hoyong | - |
dc.contributor.nonIdAuthor | Sohn, Jung-Hoon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Lignin valorization | - |
dc.subject.keywordAuthor | Enzyme secretion | - |
dc.subject.keywordAuthor | DyP-type peroxidase | - |
dc.subject.keywordAuthor | Pseudomonas putida | - |
dc.subject.keywordAuthor | Flagellar Type III secretion | - |
dc.subject.keywordPlus | VALORIZATION | - |
dc.subject.keywordPlus | SIGNAL | - |
dc.subject.keywordPlus | EXPORT | - |
dc.subject.keywordPlus | MODEL | - |
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