Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose

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dc.contributor.authorCha, Ji Wonko
dc.contributor.authorJang, Seung Hoonko
dc.contributor.authorSon, Jaewooko
dc.contributor.authorKim, Jungyeonko
dc.contributor.authorJung, Inhoko
dc.contributor.authorKim, Kyoung Heonko
dc.contributor.authorChang, Yong Keunko
dc.contributor.authorJeong, Ki Junko
dc.date.accessioned2021-11-23T06:41:40Z-
dc.date.available2021-11-23T06:41:40Z-
dc.date.created2021-11-22-
dc.date.created2021-11-22-
dc.date.created2021-11-22-
dc.date.issued2021-11-
dc.identifier.citationACS SUSTAINABLE CHEMISTRY & ENGINEERING, v.9, no.43, pp.14395 - 14404-
dc.identifier.issn2168-0485-
dc.identifier.urihttp://hdl.handle.net/10203/289367-
dc.description.abstractKlebsiella oxytoca is widely used for the biological production of 2,3-butanediol (2,3-BDO), a promising platform chemical with a broad range of applications. Here, to improve cell growth and production of 2,3-BDO under high concentration of xylose (100 g/L), we engineered K. oxytoca using an adaptive laboratory evolution and a biosensor-derived high throughput screening strategy. First, we developed a XylR-dependent xylose biosensor for the detection of intracellular xylose, and K. oxytoca containing the xylose biosensor was used for adaptive laboratory evolution in 100 g/L xylose. Cells were isolated by FACS screening, and the isolated strain (KO8S16) showed much improved cell growth with high xylose consumption rate (1.35 g/L/h) and 2,3-BDO productivity (0.53 g/L/h) compared with the wild-type strain. Through whole genome resequencing, it was revealed that a mutation in OmpR (a response regulator of osmotic stress) allowed to withstand high concentrations of xylose. Finally, fed-batch cultivation was performed by feeding high concentration of xylose, and K. oxytoca successfully produced 2,3-BDO at a concentration as high as 57.5 g/L by consuming 238.13 g/L xylose in 47 h.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.titleEngineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose-
dc.typeArticle-
dc.identifier.wosid000715216700009-
dc.identifier.scopusid2-s2.0-85116283735-
dc.type.rimsART-
dc.citation.volume9-
dc.citation.issue43-
dc.citation.beginningpage14395-
dc.citation.endingpage14404-
dc.citation.publicationnameACS SUSTAINABLE CHEMISTRY & ENGINEERING-
dc.identifier.doi10.1021/acssuschemeng.1c04118-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorChang, Yong Keun-
dc.contributor.localauthorJeong, Ki Jun-
dc.contributor.nonIdAuthorJang, Seung Hoon-
dc.contributor.nonIdAuthorKim, Jungyeon-
dc.contributor.nonIdAuthorJung, Inho-
dc.contributor.nonIdAuthorKim, Kyoung Heon-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorKlebsiella oxytoca-
dc.subject.keywordAuthor2,3-butanediol-
dc.subject.keywordAuthorxylose-
dc.subject.keywordAuthorbiosensor-
dc.subject.keywordAuthoradaptive laboratory evolution-
dc.subject.keywordPlusGCN5-RELATED N-ACETYLTRANSFERASES-
dc.subject.keywordPlusBIOMASS-DERIVED SUGARS-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusENHANCED PRODUCTION-
dc.subject.keywordPlusLIGNOCELLULOSIC BIOMASS-
dc.subject.keywordPlusFERMENTATIVE PRODUCTION-
dc.subject.keywordPlusBACILLUS-LICHENIFORMIS-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusACETYLATION-
dc.subject.keywordPlusHYDROLYSATE-
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