Sequence analysis of pseudomonas fluorescens esterase Ⅲ gene and characterization of the gene product expressed in escherichia coli = Pesudomonas fluorescens esterase Ⅲ 유전자의 Sequence와 대장균에서 발현된 산물의 순수 정제 및 특성분석

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A gene (estC) coding for a new esterase (esterase III) of Pseudomonas fluorescens was cloned into Escherichia coli JM83. DNA sequencing revealed a single open reading frame with GTG as a translation initiation codon for esterase III. This was confirmed by N-terminal amino acid sequence analysis of the purified esterase protein. The promoter region and a potential Shine-Dalgarno sequence were followed by the coding sequence of the estC gene. The amino acid sequence deduced from the nucleotide sequence cotains the consensus active site sequence, G-X-S-X-G, of serine esterase. The esterase expressed in an E. coli clone was purified by ion-exchange chromatography and gel filtration. The native form of the enzyme consisted of subunits, each with a molecular weight of 41,000. The results of substrate specificity and the inhibitor studies suggest that this enzyme is a carboxylesterase (EC 3.1.1.1) and a serine residue is present at the active site of the esterase.
Advisors
Yoo, Ook-Joonresearcher유욱준researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
1993
Identifier
68855/325007 / 000911115
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 1993.8, [ vi, 52 p.]

URI
http://hdl.handle.net/10203/28409
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68855&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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