DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yoo, Ook-Joon | - |
dc.contributor.advisor | 유욱준 | - |
dc.contributor.author | Kim, Youn-Sung | - |
dc.contributor.author | 김윤성 | - |
dc.date.accessioned | 2011-12-12T08:59:34Z | - |
dc.date.available | 2011-12-12T08:59:34Z | - |
dc.date.issued | 1993 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68855&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28409 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 1993.8, [ vi, 52 p.] | - |
dc.description.abstract | A gene (estC) coding for a new esterase (esterase III) of Pseudomonas fluorescens was cloned into Escherichia coli JM83. DNA sequencing revealed a single open reading frame with GTG as a translation initiation codon for esterase III. This was confirmed by N-terminal amino acid sequence analysis of the purified esterase protein. The promoter region and a potential Shine-Dalgarno sequence were followed by the coding sequence of the estC gene. The amino acid sequence deduced from the nucleotide sequence cotains the consensus active site sequence, G-X-S-X-G, of serine esterase. The esterase expressed in an E. coli clone was purified by ion-exchange chromatography and gel filtration. The native form of the enzyme consisted of subunits, each with a molecular weight of 41,000. The results of substrate specificity and the inhibitor studies suggest that this enzyme is a carboxylesterase (EC 3.1.1.1) and a serine residue is present at the active site of the esterase. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Sequence analysis of pseudomonas fluorescens esterase Ⅲ gene and characterization of the gene product expressed in escherichia coli | - |
dc.title.alternative | Pesudomonas fluorescens esterase Ⅲ 유전자의 Sequence와 대장균에서 발현된 산물의 순수 정제 및 특성분석 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 68855/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 000911115 | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.localauthor | 유욱준 | - |
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