Induction of cytochrome P-450S by alpha-naphthoflavone in primary cultures of adult rat hepatocytes = Alpha-naphthoflavone 에 의한 일차 배양 간세포의 cytochrome P-450 유도

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To study the induction of drug metabolism associated to cytochrome P-450 by alpha-naphthoflavone(ANF), we determined specific cytochrome P-450 dependent monooxygenase activities according to culture time and dose of ANF by using specific substrate to each cytochrome P-450, especially phenobarbital(PB)- and 3-methylcholanthrene(MC)-inducible type of P-450, and compared with activity of in two media. We found that the activity of cytochrome P-450 dependent drug metabolism was increased by the addition of ANF to culture medium. This induction of cytochrome P-450 was also idemtified by the quantitation of P-450 mRNA using typespecific oligonucleotide probe in hybridization. Also we detected the inhibition of TCDD-mediated P-450 induction by ANF(10 uM) to examine whether the induction of cytochrome P-450 by ANF occur through the cytosolic Ah receptor. In conclusion, it seems that the induction of cytochrome P-450 by ANF may be occur by the accumulation of mRNA through receptor-ligand level.
Advisors
Park, Chan-KyuresearcherYang, Kyu-Hwanresearcher박찬규researcher양규환researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1990
Identifier
67708/325007 / 000891275
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1990, [ [iv], 56 p. ]

URI
http://hdl.handle.net/10203/28371
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67708&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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