Mode of transformation of DNA-vector though E. coli cell membrane = E. coli 세포막을 통한 DNA-vector 의 형질 변환

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Mode of $CaCl_2$ mediated transformation in E. coli was studied with using PBR322 DNA and Charon4 DNA. $CaCl_2$ and heat shock was essential for transformation of E. coli. Optimal transformation occurred in 50 mM $CaCl_2$ and for 2 min of heat shock at 42$^\circ$C. Transformation occurred by heat shock with $CaCl_2$ but further incubation in ice for 30 min increased the transformation frequency. E. coli cells that heatshocked in $CaCl_2$ were transformable with foregin DNA and heat-shock of the mixture (donor DNA and recipient cells) was not essential. But heat-shock concerned in not only inducing competency but also uptake of foreign DNA. $CaCl_2$ had an effect on removal of transformation barrier of E. coli cells, rather than conformational change of donor DNA.
Advisors
Lee, Hyun-Jae이현재
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1982
Identifier
63315/325007 / 000801229
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1982.2, [ iv, 64 p. ]

URI
http://hdl.handle.net/10203/28130
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=63315&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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