DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Hyun-Jae | - |
dc.contributor.advisor | 이현재 | - |
dc.contributor.author | Lim, Dong-Bin | - |
dc.contributor.author | 임동빈 | - |
dc.date.accessioned | 2011-12-12T08:55:30Z | - |
dc.date.available | 2011-12-12T08:55:30Z | - |
dc.date.issued | 1982 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=63315&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28130 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1982.2, [ iv, 64 p. ] | - |
dc.description.abstract | Mode of $CaCl_2$ mediated transformation in E. coli was studied with using PBR322 DNA and Charon4 DNA. $CaCl_2$ and heat shock was essential for transformation of E. coli. Optimal transformation occurred in 50 mM $CaCl_2$ and for 2 min of heat shock at 42$^\circ$C. Transformation occurred by heat shock with $CaCl_2$ but further incubation in ice for 30 min increased the transformation frequency. E. coli cells that heatshocked in $CaCl_2$ were transformable with foregin DNA and heat-shock of the mixture (donor DNA and recipient cells) was not essential. But heat-shock concerned in not only inducing competency but also uptake of foreign DNA. $CaCl_2$ had an effect on removal of transformation barrier of E. coli cells, rather than conformational change of donor DNA. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Mode of transformation of DNA-vector though E. coli cell membrane | - |
dc.title.alternative | E. coli 세포막을 통한 DNA-vector 의 형질 변환 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 63315/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000801229 | - |
dc.contributor.localauthor | Lee, Hyun-Jae | - |
dc.contributor.localauthor | 이현재 | - |
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