DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Seongyong | ko |
dc.contributor.author | Kim, Dasol | ko |
dc.contributor.author | Mitchell, Robert J. | ko |
dc.contributor.author | Kim, Taesung | ko |
dc.date.accessioned | 2021-02-16T07:30:13Z | - |
dc.date.available | 2021-02-16T07:30:13Z | - |
dc.date.created | 2020-10-20 | - |
dc.date.issued | 2011-09 | - |
dc.identifier.citation | Lab on a Chip - Miniaturisation for Chemistry and Biology, v.11, no.17, pp.2916 - 2923 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | http://hdl.handle.net/10203/280773 | - |
dc.description.abstract | We present a microfabricated concentrator array device that makes it possible to quantify the predation rate of Bdellovibrio bacteriovorus, a predatory microbe, toward its prey, Escherichia coli str. MG1655. The device can accumulate both prey and predator microbes sequentially within a series of concentrator arrays using the motility of the microbes and microfabricated arrowhead-shaped ratchet structures. Since the device can constrain both prey and predator cells within 200 pL chambers at a desired range of cell densities, it was demonstrated that the device cannot only enhance the possibility of studying predation processes/cycles directly at a single cell level but can also quantify the predation rates indirectly by measuring the time-dependent fluorescent intensity signals from the prey. Furthermore, the device can produce a wide range of initial prey to predator density ratios within various concentrator arrays through the use of microfluidic mixer structures on a single array chip, which allows us to study many different conditions with a single set of cultures, and quantitatively characterize the predation behaviour/rate. Lastly, we note that this novel concentrator array device can be a very powerful tool facilitating studies of microbial predations and microbe-microbe interaction and may be broadly used in other microbial biotechnological applications. © 2011 The Royal Society of Chemistry. | - |
dc.language | English | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | A microfluidic concentrator array for quantitative predation assays of predatory microbes | - |
dc.type | Article | - |
dc.identifier.wosid | 000293651100015 | - |
dc.identifier.scopusid | 2-s2.0-80051647407 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 17 | - |
dc.citation.beginningpage | 2916 | - |
dc.citation.endingpage | 2923 | - |
dc.citation.publicationname | Lab on a Chip - Miniaturisation for Chemistry and Biology | - |
dc.identifier.doi | 10.1039/c1lc20230h | - |
dc.contributor.nonIdAuthor | Kim, Dasol | - |
dc.contributor.nonIdAuthor | Mitchell, Robert J. | - |
dc.contributor.nonIdAuthor | Kim, Taesung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | BDELLOVIBRIO-BACTERIOVORUS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BACTERIAL CHEMOTAXIS | - |
dc.subject.keywordPlus | SURVIVAL STRATEGY | - |
dc.subject.keywordPlus | SWIMMING BACTERIA | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | HOST BACTERIA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | GRADIENTS | - |
dc.subject.keywordPlus | MICROBIOLOGY | - |
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