Regulation of apoptotic Mst1 kinase activity by RASSF1A in vivo = 세포 사멸 과정 중 RASSF1A의 Mst1 카이네이즈 활성 조절에 관한 연구

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Mst1 (mammalian sterile20-like kinase 1) is activated by both caspase-mediated cleavage and phosphorylation during apoptosis induced by Fas/CD95, staurosporine or genotoxic agents. However, the physiological regulator of Mst1 has not been identified. In the present study, we demonstrate that Mst1 is regulated by the tumor suppressor RASSF1A which regulates mitosis by inhibiting APC/C and induces a prolonged G2/M arrest followed by apoptosis. We show that Mst1 interacts with RASSF1A and they colocalize to microtubules throughout the cell cycle. While the activity of Mst1 is inhibited by direct addition of purified RASSF1A in vitro, overexpression of RASSF1A augments the kinase activity of Mst1 in mammalian cells, suggesting that in vivo regulation of Mst1 by RASSF1A is more complex than simple association between them. Moreover, both apoptosis and Mst1 activation are significantly suppressed in RASSF1A-depleted cells, suggesting that RASSF1A induces the activation of Mst1 during apoptosis. Overall, we propose that RASSF1A is required for Mst1 activation and contributes to the apoptotic function of Mst1 by regulating the Mst1 kinase activity in vivo.
Advisors
Lim, Dae-Silresearcher임대식researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
243522/325007  / 020033391
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2005.2, [ iv, 37 p. ]

Keywords

RASSF1A; Mst1; apoptosis; 카이네이즈 활성서의 수모일레이션 분석 연구廢?분석; 세포 사멸; kinase of on-chip SUMOylation provides a strategy enabling a high throughput analysis of SUMO conjugation

URI
http://hdl.handle.net/10203/28048
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243522&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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