DC Field | Value | Language |
---|---|---|
dc.contributor.author | Moon, Jeong | ko |
dc.contributor.author | Kwon, Hyung-Jun | ko |
dc.contributor.author | Yong, Dongeun | ko |
dc.contributor.author | Lee, In-Chul | ko |
dc.contributor.author | Kim, Hongki | ko |
dc.contributor.author | Kang, Hyunju | ko |
dc.contributor.author | Lim, Eun-Kyung | ko |
dc.contributor.author | Lee, Kyu-Sun | ko |
dc.contributor.author | Jung, Juyeon | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.contributor.author | Kang, Taejoon | ko |
dc.date.accessioned | 2021-01-05T07:30:06Z | - |
dc.date.available | 2021-01-05T07:30:06Z | - |
dc.date.created | 2020-12-08 | - |
dc.date.created | 2020-12-08 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.citation | ACS SENSORS, v.5, no.12, pp.4017 - 4026 | - |
dc.identifier.issn | 2379-3694 | - |
dc.identifier.uri | http://hdl.handle.net/10203/279501 | - |
dc.description.abstract | Viruses have been a continuous threat to human beings. The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a pandemic that is still ongoing worldwide. Previous pandemic influenza A virus (pH1N1) might be re-emerging through a drug-resistant mutation. We report a colorimetric viral detection method based on the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 endonuclease dead (dCas9) system. In this method, RNA in the viral lysate was directly recognized by the CRISPR/dCas9 system with biotin-protospacer adjacent motif (PAM)-presenting oligonucleotide (PAMmer). Streptavidin-horseradish peroxidase then bound to biotin-PAMmer, inducing a color change through the oxidation of 3,3′,5,5′-tetramethylbenzidine. Using the developed method, we successfully identified SARS-CoV-2, pH1N1, and pH1N1/H275Y viruses by the naked eye. Moreover, the detection of viruses in human nasopharyngeal aspirates and sputum was demonstrated. Finally, clinical samples from COVID-19 patients led to a successful diagnosis. We anticipate that the current method can be employed for simple and accurate diagnosis of viruses. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Colorimetric Detection of SARS-CoV-2 and Drug-Resistant pH1N1 Using CRISPR/dCas9 | - |
dc.type | Article | - |
dc.identifier.wosid | 000603403600032 | - |
dc.identifier.scopusid | 2-s2.0-85097778036 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 4017 | - |
dc.citation.endingpage | 4026 | - |
dc.citation.publicationname | ACS SENSORS | - |
dc.identifier.doi | 10.1021/acssensors.0c01929 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Kwon, Hyung-Jun | - |
dc.contributor.nonIdAuthor | Yong, Dongeun | - |
dc.contributor.nonIdAuthor | Lee, In-Chul | - |
dc.contributor.nonIdAuthor | Kim, Hongki | - |
dc.contributor.nonIdAuthor | Kang, Hyunju | - |
dc.contributor.nonIdAuthor | Lim, Eun-Kyung | - |
dc.contributor.nonIdAuthor | Lee, Kyu-Sun | - |
dc.contributor.nonIdAuthor | Jung, Juyeon | - |
dc.contributor.nonIdAuthor | Kang, Taejoon | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | COVID-19 | - |
dc.subject.keywordAuthor | drug-resistance | - |
dc.subject.keywordAuthor | influenza virus | - |
dc.subject.keywordAuthor | CRISPR/dCas9 | - |
dc.subject.keywordAuthor | colorimetry | - |
dc.subject.keywordPlus | INFLUENZA-A H1N1 | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | DIAGNOSTICS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SYSTEMS | - |
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