DC Field | Value | Language |
---|---|---|
dc.contributor.author | Heo, Inha | ko |
dc.contributor.author | Ha, Minju | ko |
dc.contributor.author | Lim, Jaechul | ko |
dc.contributor.author | Yoon, Mi-Jeong | ko |
dc.contributor.author | Park, Jong-Eun | ko |
dc.contributor.author | Kwon, S. Chul | ko |
dc.contributor.author | Chang, Hyeshik | ko |
dc.contributor.author | Kim, V. Narry | ko |
dc.date.accessioned | 2020-12-04T00:50:15Z | - |
dc.date.available | 2020-12-04T00:50:15Z | - |
dc.date.created | 2020-12-04 | - |
dc.date.created | 2020-12-04 | - |
dc.date.issued | 2012-10 | - |
dc.identifier.citation | CELL, v.151, no.3, pp.521 - 532 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/10203/278029 | - |
dc.description.abstract | RNase III Drosha initiates microRNA (miRNA) maturation by cleaving a primary miRNA transcript and releasing a pre-miRNA with a 2 nt 3' overhang. Dicer recognizes the 2 nt 3' overhang structure to selectively process pre-miRNAs. Here, we find that, unlike prototypic pre-miRNAs (group I), group II pre-miRNAs acquire a shorter (1 nt) 3' overhang from Drosha processing and therefore require a 3'-end mono-uridylation for Dicer processing. The majority of let-7 and miR-105 belong to group II. We identify TUT7/ZCCHC6, TUT4/ZCCHC11, and TUT2/PAPD4/GLD2 as the terminal uridylyl transferases responsible for pre-miRNA mono-uridylation. The TUTs act specifically on dsRNAs with a 1 nt 3' overhang, thereby creating a 2 nt 3' overhang. Depletion of TUTs reduces let-7 levels and disrupts let-7 function. Although the let-7 suppressor, Lin28, induces inhibitory oligo-uridylation in embryonic stem cells, mono-uridylation occurs in somatic cells lacking Lin28 to promote let-7 biogenesis. Our study reveals functional duality of uridylation and introduces TUT7/4/2 as components of the miRNA biogenesis pathway. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | Mono-Uridylation of Pre-MicroRNA as a Key Step in the Biogenesis of Group II let-7 MicroRNAs | - |
dc.type | Article | - |
dc.identifier.wosid | 000310529300010 | - |
dc.identifier.scopusid | 2-s2.0-84868153864 | - |
dc.type.rims | ART | - |
dc.citation.volume | 151 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 521 | - |
dc.citation.endingpage | 532 | - |
dc.citation.publicationname | CELL | - |
dc.identifier.doi | 10.1016/j.cell.2012.09.022 | - |
dc.contributor.localauthor | Park, Jong-Eun | - |
dc.contributor.nonIdAuthor | Heo, Inha | - |
dc.contributor.nonIdAuthor | Ha, Minju | - |
dc.contributor.nonIdAuthor | Lim, Jaechul | - |
dc.contributor.nonIdAuthor | Yoon, Mi-Jeong | - |
dc.contributor.nonIdAuthor | Kwon, S. Chul | - |
dc.contributor.nonIdAuthor | Chang, Hyeshik | - |
dc.contributor.nonIdAuthor | Kim, V. Narry | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RNA-INTERFERENCE | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | POLY(A) POLYMERASES | - |
dc.subject.keywordPlus | NUCLEAR EXPORT | - |
dc.subject.keywordPlus | MICROPROCESSOR COMPLEX | - |
dc.subject.keywordPlus | CAENORHABDITIS-ELEGANS | - |
dc.subject.keywordPlus | DROSHA-DGCR8 COMPLEX | - |
dc.subject.keywordPlus | MOLECULAR-BASIS | - |
dc.subject.keywordPlus | 3&apos | - |
dc.subject.keywordPlus | ADENYLATION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
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