Xylitol production with high yield by xylitol dehydrogenase gene-disrupted mutant of candida tropicalis = Xylitol Dehydrogenase 유전자가 제거된 Candida tropicalis 변이주를 이용한 고수율의 Xylitol 생산

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A gene encoding xylitol dehydrogenase (XDH) of Candida tropicalis ATCC 20913 was cloned and characterized. The 1,095-bp coding sequence of the XYL2 gene encodes a polypeptide of 364 amino acids, with a molecular mass of 39.4 kDa. The XYL2 gene of Candida tropicalis was disrupted by sequential gene replacement using Ura-blasting method. The XYL2-disrupted mutant, BSXDH-3, could not grow on a minimal medium containing D-xylose as a sole carbon source. Enzyme assay experiment indicated that BSXDH-3 lost apparently all XDH activity. Xylitol production by BSXDH-3 was evaluated using xylitol fermentation medium containing glucose as a co-substrate. Since glucose was not sufficient as a co-substrate, various carbon sources were screened for efficient cofactor regeneration. Glycerol was selected as the best co-substrate and, subsequently, the optimization of fermentation condition was carried out. As the result of experiments, BSXDH-3 produced xylitol with a volumetric productivity of 3.23 $g1^{-1}h^{-1}$ a specific productivity of 0.76 g$g1^{-1}h^{-1}$, and a xylitol yield of 98 %. This is the first report of gene disruption of C. tropicalis for enhancing the efficiency of xylitol production and will be a turning point of process development for xylitol production.
Advisors
Kim, Jung-Hoeresearcher김정회researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2006
Identifier
254176/325007  / 020005012
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2006.2, [ vii, 92 p. ]

Keywords

Candida tropicalis; xylitol dehydrogenase; xylitol; gene disruption; 유전자 제거; 캔다다 트로피칼리스; 자일리톨 탈수소효소; 자일리톨

URI
http://hdl.handle.net/10203/27599
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=254176&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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