DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Jung-Hoe | - |
dc.contributor.advisor | 김정회 | - |
dc.contributor.author | Ko, Byoung-Sam | - |
dc.contributor.author | 고병삼 | - |
dc.date.accessioned | 2011-12-12T07:54:36Z | - |
dc.date.available | 2011-12-12T07:54:36Z | - |
dc.date.issued | 2006 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=254176&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27599 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2006.2, [ vii, 92 p. ] | - |
dc.description.abstract | A gene encoding xylitol dehydrogenase (XDH) of Candida tropicalis ATCC 20913 was cloned and characterized. The 1,095-bp coding sequence of the XYL2 gene encodes a polypeptide of 364 amino acids, with a molecular mass of 39.4 kDa. The XYL2 gene of Candida tropicalis was disrupted by sequential gene replacement using Ura-blasting method. The XYL2-disrupted mutant, BSXDH-3, could not grow on a minimal medium containing D-xylose as a sole carbon source. Enzyme assay experiment indicated that BSXDH-3 lost apparently all XDH activity. Xylitol production by BSXDH-3 was evaluated using xylitol fermentation medium containing glucose as a co-substrate. Since glucose was not sufficient as a co-substrate, various carbon sources were screened for efficient cofactor regeneration. Glycerol was selected as the best co-substrate and, subsequently, the optimization of fermentation condition was carried out. As the result of experiments, BSXDH-3 produced xylitol with a volumetric productivity of 3.23 $g1^{-1}h^{-1}$ a specific productivity of 0.76 g$g1^{-1}h^{-1}$, and a xylitol yield of 98 %. This is the first report of gene disruption of C. tropicalis for enhancing the efficiency of xylitol production and will be a turning point of process development for xylitol production. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Candida tropicalis | - |
dc.subject | xylitol dehydrogenase | - |
dc.subject | xylitol | - |
dc.subject | gene disruption | - |
dc.subject | 유전자 제거 | - |
dc.subject | 캔다다 트로피칼리스 | - |
dc.subject | 자일리톨 탈수소효소 | - |
dc.subject | 자일리톨 | - |
dc.title | Xylitol production with high yield by xylitol dehydrogenase gene-disrupted mutant of candida tropicalis | - |
dc.title.alternative | Xylitol Dehydrogenase 유전자가 제거된 Candida tropicalis 변이주를 이용한 고수율의 Xylitol 생산 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 254176/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020005012 | - |
dc.contributor.localauthor | Kim, Jung-Hoe | - |
dc.contributor.localauthor | 김정회 | - |
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