Characterization of recombinant CHO cells in the course of gene amplification = 재조합 CHO 세포에서 유전자 증폭과정의 특성 연구

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Chinese hamster ovary cell (CHO) production of humanized antibody was increased by amplification of cotransfected glutamine synthetase (GS) gene using additional rounds of selection with GS inhibitor methionine sulfoximine (MSX). The highest producer (HP) subclones were isolated from each MSX level and was characterized with respect to antibody production, cell growth rate, cell size and mitochondria content. HP subclones showed variable productivities, copy numbers and cellular characteristics that didn’t increase concomitantly to MSX increase. Generally, low production stability was observed during long-term culture. Presence of selection pressure didn’t prevent the drastic productivity drop observed within 10 generations during the long-term culture. Addition of glutamine enhanced the productivity and higher accumulative antibody yields were obtained in glutamine containing media, compared to the glutamine-free media. Significant decrease of copy numbers was observed, but the prod
Advisors
Lee, Gyun-Minresearcher이균민researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
244499/325007  / 000955341
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2005.2, [ x, 135 p. ]

Keywords

fish; dihydrofolate reductase; glutamine synthetase; gene amplification; CHO cells; telomere; 텔로미어; FISH; 다이하이드로폴레이트 환원효소; 글루타민 합성효소; 유전자 증폭; CHO 세포

URI
http://hdl.handle.net/10203/27582
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=244499&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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