This paper presents a new fuzzy Lyapunov function (FLF) for the stability analysis of continuous-time Takagi-Sugeno (T-S) fuzzy systems. Unlike conventional FLFs, the proposed one depends not only on the fuzzy weighting functions of the T-S fuzzy systems but on their first-order time derivatives as well. Based on the proposed FLF, a sufficient stability condition is derived in the form of linear matrix inequalities, depending on the upper bounds on the second-order time derivative of the fuzzy weighting functions, as well as the first-order ones. Finally, some examples demonstrate that the proposed condition can provide less conservative results than the previous ones in the literature.