DC Field | Value | Language |
---|---|---|
dc.contributor.author | KIM, BS | ko |
dc.contributor.author | LEE, SC | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.contributor.author | Chang, YongKeun | ko |
dc.contributor.author | Woo, Seong-Ihl | ko |
dc.date.accessioned | 2019-03-06T19:35:03Z | - |
dc.date.available | 2019-03-06T19:35:03Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1994-07 | - |
dc.identifier.citation | ENZYME AND MICROBIAL TECHNOLOGY, v.16, no.7, pp.556 - 561 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250734 | - |
dc.description.abstract | Alcaligenes eutrophus NCIMB 11599 was cultivated to produce poly(3-hydroxybutyric-co-3-hydroxyvaleric acid), P(3HB-co-3HV), from glucose and propionic acid by a two-stage, fed-batch culture technique. The glucose concentration of the culture broth was controlled at 10-20 gl(-1) using an on-line glucose analyzer during the whole culture period. Nitrogen limitation was applied at a cell concentration of 60-70 gl(-1), when the glucose feed was replaced by the mixture of glucose and propionic acid. The effect of the ratio of propionic acid to glucose (P/G ratio) in the feed on the copolymer production was examined. The final copolymer concentrations of 117, 74, and 64 gl(-1), polymer contents of 74%, 57%, and 56.5% of dry cell weight, and productivities of 2.55, 1.67, and 1.64 gl(-1) h (-1) were obtained when the P/G ratios in the feed were 0.17, 0.35, and 0.52 (mol propionic acid mol(-1) glucose), respectively, showing reduced production with increasing P/G ratio. However, the 3-hydroxyvaleric acid (3HV) fraction in the copolymer increased with increasing P/G ratio, resulting in 14.3 mol% of 3HV at the P/G ratio of 0.52 (mol mol(-1)). The concentration of propionic acid in the culture broth was maintained below 1.3 gl(-1) when the P/G ratio in the feed was 0.17 or 0.35 (mol mol(-1)), but increased gradually when using the ratio of 0.52 (mol mol(-1)). | - |
dc.language | English | - |
dc.publisher | BUTTERWORTH-HEINEMANN | - |
dc.title | Production of poly-(3-hydroxybutyric-co-hydroxyvaleric acid) by fed-batch culture of Alcaligenes eutrophus with substrate feeding using on-line glucose analyzer | - |
dc.type | Article | - |
dc.identifier.wosid | A1994NR96600003 | - |
dc.identifier.scopusid | 2-s2.0-0028180288 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 556 | - |
dc.citation.endingpage | 561 | - |
dc.citation.publicationname | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.localauthor | Chang, YongKeun | - |
dc.contributor.localauthor | Woo, Seong-Ihl | - |
dc.contributor.nonIdAuthor | KIM, BS | - |
dc.contributor.nonIdAuthor | LEE, SC | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | POLY(3-HYDROXYBUTYRIC-CO-3-HYDROXYVALERIC ACID) | - |
dc.subject.keywordAuthor | P(3HB-CO-3HV) | - |
dc.subject.keywordAuthor | ALCALIGENES EUTROPHUS | - |
dc.subject.keywordAuthor | HIGH CELL DENSITY CULTURE | - |
dc.subject.keywordAuthor | FED-BATCH CULTURE | - |
dc.subject.keywordAuthor | ONLINE GLUCOSE ANALYZER | - |
dc.subject.keywordAuthor | SUBSTRATE CONCENTRATION CONTROL | - |
dc.subject.keywordPlus | BETA-HYDROXYBUTYRIC ACID | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | MASS-PRODUCTION | - |
dc.subject.keywordPlus | 3-HYDROXYBUTYRATE | - |
dc.subject.keywordPlus | 3-HYDROXYVALERATE | - |
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