DC Field | Value | Language |
---|---|---|
dc.contributor.author | Noh, JM | ko |
dc.contributor.author | Sung, SW | ko |
dc.contributor.author | Jeon, MK | ko |
dc.contributor.author | Kim, SH | ko |
dc.contributor.author | Lee, LP | ko |
dc.contributor.author | Woo, Seong-Ihl | ko |
dc.date.accessioned | 2019-03-06T10:16:03Z | - |
dc.date.available | 2019-03-06T10:16:03Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-08 | - |
dc.identifier.citation | SENSORS AND ACTUATORS A-PHYSICAL, v.122, no.2, pp.196 - 202 | - |
dc.identifier.issn | 0924-4247 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250616 | - |
dc.description.abstract | A simple, reliable liquid crystal (LC) thermometry of a polymerase chain reaction (PCR) chip that has a microchamber inside the chip and a film heater and a film sensor patterned on the surface is firstly introduced. The silicon-based PCR chip is made by MEMS technology. A non-linear feedback proportional-integral control scheme adjusts the power input of the Pt heater to control the surface temperature of the micro-PCR chip measured by the Pt sensor with an accuracy of less than +/- 0.1 degrees C at steady state. We use the LC thermometry to analyze the temperature distribution in the microchamber of the micro-PCR chip. The reflected colors of LC's inside the microchamber are captured through a charge-coupled device (CCD) camera, subsequently analyzed using a hue-based color analysis system. Two LC's of which individual working range is near the typical PCR temperature of 55 degrees C and 72 degrees C are calibrated in a constant temperature bath with a resolution of less than +/- 0.3 degrees C. While the average microchamber temperature near the annealing temperature is similar to the surface temperature, it is lower than the surface temperature by ca. 1 degrees C in the range of the extension temperature. Also, the temperature variations along the center of the microchamber, are less than 1.5 +/- 0.5 degrees C and 3 +/- 0.3 degrees C in the range of the annealing and the extension temperature, respectively. (C) 2005 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject | POLYMERASE-CHAIN-REACTION | - |
dc.subject | ELECTROPHORESIS CAPILLARIES | - |
dc.subject | THERMOMETRY | - |
dc.subject | AMPLIFICATION | - |
dc.subject | DEVICE | - |
dc.subject | CHIP | - |
dc.title | In situ thermal diagnostics of the micro-PCR system using liquid crystals | - |
dc.type | Article | - |
dc.identifier.wosid | 000231347200004 | - |
dc.identifier.scopusid | 2-s2.0-23344432646 | - |
dc.type.rims | ART | - |
dc.citation.volume | 122 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 196 | - |
dc.citation.endingpage | 202 | - |
dc.citation.publicationname | SENSORS AND ACTUATORS A-PHYSICAL | - |
dc.identifier.doi | 10.1016/j.sna.2005.05.008 | - |
dc.contributor.localauthor | Woo, Seong-Ihl | - |
dc.contributor.nonIdAuthor | Noh, JM | - |
dc.contributor.nonIdAuthor | Sung, SW | - |
dc.contributor.nonIdAuthor | Jeon, MK | - |
dc.contributor.nonIdAuthor | Kim, SH | - |
dc.contributor.nonIdAuthor | Lee, LP | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | liquid crystals | - |
dc.subject.keywordAuthor | PCR chip | - |
dc.subject.keywordAuthor | microfabrication | - |
dc.subject.keywordAuthor | temperature uniformity | - |
dc.subject.keywordAuthor | hue | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | ELECTROPHORESIS CAPILLARIES | - |
dc.subject.keywordPlus | THERMOMETRY | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | CHIP | - |
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