DC Field | Value | Language |
---|---|---|
dc.contributor.author | Piatkevich, Kiryl D. | ko |
dc.contributor.author | Jung, Erica E. | ko |
dc.contributor.author | Straub, Christoph | ko |
dc.contributor.author | Linghu, Changyang | ko |
dc.contributor.author | Park, Demian | ko |
dc.contributor.author | Suk, Ho-Jun | ko |
dc.contributor.author | Hochbaum, Daniel R. | ko |
dc.contributor.author | Goodwin, Daniel | ko |
dc.contributor.author | Pnevmatikakis, Eftychios | ko |
dc.contributor.author | Pak, Nikita | ko |
dc.contributor.author | Kawashima, Takashi | ko |
dc.contributor.author | Yang, Chao-Tsung | ko |
dc.contributor.author | Rhoades, Jeffrey L. | ko |
dc.contributor.author | Shemesh, Or | ko |
dc.contributor.author | Asano, Shoh | ko |
dc.contributor.author | Yoon, Young-Gyu | ko |
dc.contributor.author | Freifeld, Limor | ko |
dc.contributor.author | Saulnier, Jessica L. | ko |
dc.contributor.author | Riegler, Clemens | ko |
dc.contributor.author | Engert, Florian | ko |
dc.contributor.author | Hughes, Thom | ko |
dc.contributor.author | Drobizhev, Mikhail | ko |
dc.contributor.author | Szabo, Balint | ko |
dc.contributor.author | Ahrens, Misha B. | ko |
dc.contributor.author | Flavell, Steven W. | ko |
dc.contributor.author | Sabatini, Bernardo L. | ko |
dc.contributor.author | Boyden, Edward S. | ko |
dc.date.accessioned | 2018-11-22T07:10:32Z | - |
dc.date.available | 2018-11-22T07:10:32Z | - |
dc.date.created | 2018-11-16 | - |
dc.date.created | 2018-11-16 | - |
dc.date.created | 2018-11-16 | - |
dc.date.issued | 2018-04 | - |
dc.identifier.citation | NATURE CHEMICAL BIOLOGY, v.14, no.4, pp.352 - + | - |
dc.identifier.issn | 1552-4450 | - |
dc.identifier.uri | http://hdl.handle.net/10203/246956 | - |
dc.description.abstract | We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing proteins that simultaneously exhibit several specific properties, we can screen hundreds of thousands of proteins in a library in just a few hours, evaluating each along multiple performance axes. To demonstrate the power of this approach, we created a genetically encoded fluorescent voltage indicator, simultaneously optimizing its brightness and membrane localization using our microscopy-guided cell-picking strategy. We produced the high-performance opsin-based fluorescent voltage reporter Archon1 and demonstrated its utility by imaging spiking and millivolt-scale subthreshold and synaptic activity in acute mouse brain slices and in larval zebrafish in vivo. We also measured postsynaptic responses downstream of optogenetically controlled neurons in C. elegans. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters | - |
dc.type | Article | - |
dc.identifier.wosid | 000428522900010 | - |
dc.identifier.scopusid | 2-s2.0-85042540110 | - |
dc.type.rims | ART | - |
dc.citation.volume | 14 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 352 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | NATURE CHEMICAL BIOLOGY | - |
dc.identifier.doi | 10.1038/s41589-018-0004-9 | - |
dc.contributor.localauthor | Yoon, Young-Gyu | - |
dc.contributor.nonIdAuthor | Piatkevich, Kiryl D. | - |
dc.contributor.nonIdAuthor | Jung, Erica E. | - |
dc.contributor.nonIdAuthor | Straub, Christoph | - |
dc.contributor.nonIdAuthor | Linghu, Changyang | - |
dc.contributor.nonIdAuthor | Park, Demian | - |
dc.contributor.nonIdAuthor | Suk, Ho-Jun | - |
dc.contributor.nonIdAuthor | Hochbaum, Daniel R. | - |
dc.contributor.nonIdAuthor | Goodwin, Daniel | - |
dc.contributor.nonIdAuthor | Pnevmatikakis, Eftychios | - |
dc.contributor.nonIdAuthor | Pak, Nikita | - |
dc.contributor.nonIdAuthor | Kawashima, Takashi | - |
dc.contributor.nonIdAuthor | Yang, Chao-Tsung | - |
dc.contributor.nonIdAuthor | Rhoades, Jeffrey L. | - |
dc.contributor.nonIdAuthor | Shemesh, Or | - |
dc.contributor.nonIdAuthor | Asano, Shoh | - |
dc.contributor.nonIdAuthor | Freifeld, Limor | - |
dc.contributor.nonIdAuthor | Saulnier, Jessica L. | - |
dc.contributor.nonIdAuthor | Riegler, Clemens | - |
dc.contributor.nonIdAuthor | Engert, Florian | - |
dc.contributor.nonIdAuthor | Hughes, Thom | - |
dc.contributor.nonIdAuthor | Drobizhev, Mikhail | - |
dc.contributor.nonIdAuthor | Szabo, Balint | - |
dc.contributor.nonIdAuthor | Ahrens, Misha B. | - |
dc.contributor.nonIdAuthor | Flavell, Steven W. | - |
dc.contributor.nonIdAuthor | Sabatini, Bernardo L. | - |
dc.contributor.nonIdAuthor | Boyden, Edward S. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NEURONAL-ACTIVITY | - |
dc.subject.keywordPlus | MICROBIAL RHODOPSIN | - |
dc.subject.keywordPlus | SPINAL-CORD | - |
dc.subject.keywordPlus | ZEBRAFISH | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | TRANSGENESIS | - |
dc.subject.keywordPlus | NEUROSCIENCE | - |
dc.subject.keywordPlus | EXCITATION | - |
dc.subject.keywordPlus | MICROSCOPY | - |
dc.subject.keywordPlus | CELLS | - |
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