Protein-Patterning on Functionalized, Non-Biofouling Poly[N-acryloxysuccinimide-co-oligo(ethylene glycol) methyl ether methacrylate] Film-Coated PET Surfaces

Abstract

We successfully fabricated poly(ethylene terephthalate) (PET) surfaces through a perfluoroaryl azide-based photochemical reaction, and subsequently formed an intrinsically activated, non-biofouling poly[N-acryloxysuccinimide-co-oligo(ethylene glycol) methyl ether methacrylate] on the surface through surface-initiated, controlled radical polymerization. The grafted copolymer film on PET facilely generated a protein pattern using a microcontact printing technique without employing both an activation step to introduce an active functional group (e.g., succinimidyl ester) and a passivation process for minimizing non-specific adsorption. Consequently, we characterized the functionalized PET surfaces by using various methods including contact angle measurement, X-ray photoelectron spectroscopy (XPS), scanning probe microscopy (SPM), field-emission scanning electron microscopy (FE-SEM). In addition, we evaluated the non-biofouling efficacy of the protein-patterned copolymer film on PET by confocal laser scanning microscopy.