DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Seunghoon | ko |
dc.contributor.author | Cha, Ji Won | ko |
dc.contributor.author | Han, Nam Soo | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2018-07-24T02:23:19Z | - |
dc.date.available | 2018-07-24T02:23:19Z | - |
dc.date.created | 2018-07-04 | - |
dc.date.created | 2018-07-04 | - |
dc.date.created | 2018-07-04 | - |
dc.date.created | 2018-07-04 | - |
dc.date.issued | 2018-06 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.8, pp.8852 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/244031 | - |
dc.description.abstract | The lactic acid bacteria (LAB) Leuconostoc citreum are non-sporulating hetero-fermentative bacteria that play an important role in the fermented food industry. In this study, for the enhanced and reliable production of recombinant proteins in L. citreum, we developed a bicistronic design (BCD) expression system which includes a short leader peptide (1st cistron) followed by target genes (2nd cistron) under the control of a single promoter. Using superfolder green fluorescent protein (sfGFP) as a reporter, the functionality of BCD in L. citreum was verified. Further, to improve the expression in BCD, we tried to engineer a Shine-Dalgarno sequence (SD2) for the 2nd cistron and a promoter by FACS screening of random libraries, and both strong SD2 (eSD2) and promoter (P-710V4) were successfully isolated. The usefulness of the engineered BCD with P-710V4 and eSD2 was further validated using three model proteins-glutathione-s-transferase, human growth hormone, and alpha-amylase. All examined proteins were successfully produced with levels highly increased compared with those in the original BCD as well as the monocistronic design (MCD) expression system. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Development of bicistronic expression system for the enhanced and reliable production of recombinant proteins in Leuconostoc citreum | - |
dc.type | Article | - |
dc.identifier.wosid | 000434776600058 | - |
dc.identifier.scopusid | 2-s2.0-85048446001 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.beginningpage | 8852 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/s41598-018-27091-z | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Han, Nam Soo | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | LACTIC-ACID BACTERIA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | TRANSLATION INITIATION | - |
dc.subject.keywordPlus | LACTOCOCCUS-LACTIS | - |
dc.subject.keywordPlus | D-LACTATE | - |
dc.subject.keywordPlus | LEVEL | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | LACTIC-ACID BACTERIA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | TRANSLATION INITIATION | - |
dc.subject.keywordPlus | LACTOCOCCUS-LACTIS | - |
dc.subject.keywordPlus | D-LACTATE | - |
dc.subject.keywordPlus | LEVEL | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
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