DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyeonsoo | ko |
dc.contributor.author | Yang, Jeongmo | ko |
dc.contributor.author | Cho, Sukhyeong | ko |
dc.contributor.author | Jeong, Kijun | ko |
dc.contributor.author | Park, Jinbyung | ko |
dc.contributor.author | Lee, Jinwon | ko |
dc.date.accessioned | 2018-04-24T06:34:06Z | - |
dc.date.available | 2018-04-24T06:34:06Z | - |
dc.date.created | 2018-04-18 | - |
dc.date.created | 2018-04-18 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.citation | PROCESS BIOCHEMISTRY, v.66, pp.61 - 69 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://hdl.handle.net/10203/241430 | - |
dc.description.abstract | The conversion of ricinoleic acid from renewable sources to long-chain am-dicarboxylic acids or w-hydroxyl carboxylic acids by microbial processes is constrained by toxicity issues. Here, we demonstrate the possible role of Corynebacterium glutamicum as a new microbial strategy for the biotransformation of fatty acids. The established strain Escherichia coli failed to grow at 5 mM n-heptanoic acid, while the specific growth rate of C. glutamicum declined by 28%. We partially constructed a previously designed multistep biocatalytic pathway in C. glutamicum, and confirmed that the C. glutamicum biocatalyst successfully converted ricinoleic acid to undec-9-enoic acid, heptyl ester via 12-keto-oleic acid. We investigated the effects of cultivation and reaction temperatures, and the type and concentration of non-ionic detergent on recombinant C. glutamicum whole-cell bioconversion. At a cultivation temperature of 30 degrees C and a reaction temperature of 35 degrees C, and in the presence of 0.09 g/L Triton X-100, the whole-cell C. glutamicum biocatalyst produced 0.8 mM undec-9-enoic acid, heptyl ester from 1.9 mM 12-ketooleic acid. It also generated 0.7 mM undec-9-enoic acid, heptyl ester from 5.5 mM ricinoleic acid. This is the first report of undec-9-enoic acid, heptyl ester production using a recombinant C. glutamicum-based biocatalyst. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | BAEYER-VILLIGER MONOOXYGENASES | - |
dc.subject | RICINOLEIC ACID | - |
dc.subject | EXPRESSION | - |
dc.subject | BIOTRANSFORMATION | - |
dc.subject | BIOCONVERSION | - |
dc.subject | DETERGENTS | - |
dc.subject | PROMOTERS | - |
dc.subject | CLONING | - |
dc.subject | FUSION | - |
dc.subject | ENZYME | - |
dc.title | Microbial synthesis of undec-9-enoic acid, heptyl ester from renewable fatty acids using recombinant Corynebacterium glutamicum-based whole-cell biocatalyst | - |
dc.type | Article | - |
dc.identifier.wosid | 000427668800006 | - |
dc.identifier.scopusid | 2-s2.0-85039805553 | - |
dc.type.rims | ART | - |
dc.citation.volume | 66 | - |
dc.citation.beginningpage | 61 | - |
dc.citation.endingpage | 69 | - |
dc.citation.publicationname | PROCESS BIOCHEMISTRY | - |
dc.identifier.doi | 10.1016/j.procbio.2017.12.009 | - |
dc.contributor.localauthor | Jeong, Kijun | - |
dc.contributor.nonIdAuthor | Kim, Hyeonsoo | - |
dc.contributor.nonIdAuthor | Yang, Jeongmo | - |
dc.contributor.nonIdAuthor | Cho, Sukhyeong | - |
dc.contributor.nonIdAuthor | Park, Jinbyung | - |
dc.contributor.nonIdAuthor | Lee, Jinwon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Biotransformation | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | Undec-9-enoic acid | - |
dc.subject.keywordAuthor | Heptyl ester | - |
dc.subject.keywordAuthor | Renewable fatty acid | - |
dc.subject.keywordPlus | BAEYER-VILLIGER MONOOXYGENASES | - |
dc.subject.keywordPlus | RICINOLEIC ACID | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BIOTRANSFORMATION | - |
dc.subject.keywordPlus | BIOCONVERSION | - |
dc.subject.keywordPlus | DETERGENTS | - |
dc.subject.keywordPlus | PROMOTERS | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | FUSION | - |
dc.subject.keywordPlus | ENZYME | - |
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