DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Ye-Lim | ko |
dc.contributor.author | Lee, Chang Yeol | ko |
dc.contributor.author | Park, Jung-Hun | ko |
dc.contributor.author | Park, Ki Soo | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2018-04-24T04:26:27Z | - |
dc.date.available | 2018-04-24T04:26:27Z | - |
dc.date.created | 2018-04-02 | - |
dc.date.created | 2018-04-02 | - |
dc.date.created | 2018-04-02 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.citation | NANOSCALE, v.10, no.9, pp.4339 - 4343 | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.uri | http://hdl.handle.net/10203/241217 | - |
dc.description.abstract | A simple, colorimetric method is developed for the determination of deoxyribonuclease I (DNase I) activity based on the novel finding that DNase I can promote the photoinduced synthesis of gold nanoparticles (AuNPs). In the absence of DNase I, a phosphorothioate (PS) DNA probe remains intact and captures Au(III) through a strong Au-thiol interaction, which prevents the photoinduced synthesis of AuNPs, leaving the sample in a colorless state. On the other hand, in the presence of DNase I, the PS DNA probe is cleaved into small fragments that are removed via a simple purification process. The resulting solution, after the incubation with HAuCl4 and threonine (Thr), forms AuNPs by UV light irradiation with the aid of Thr which acts as a catalyst for the Au(III) reduction process. As a result, a red-colored suspension is produced. By monitoring the color changes of the samples with the naked eye, the DNase I activity was conveniently determined. In addition, the clinical utility of this simple, yet highly efficient colorimetric strategy was verified by reliably quantifying the DNase I activities in a bovine urine sample. Importantly, the working principle designed for the determination of DNase I activity was successfully expanded for the detection of target nucleic acids, ensuring the universal applicability of the developed assay system. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | A signal-on, colorimetric determination of deoxyribonuclease I activity utilizing the photoinduced synthesis of gold nanoparticles | - |
dc.type | Article | - |
dc.identifier.wosid | 000426708500021 | - |
dc.identifier.scopusid | 2-s2.0-85042874784 | - |
dc.type.rims | ART | - |
dc.citation.volume | 10 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 4339 | - |
dc.citation.endingpage | 4343 | - |
dc.citation.publicationname | NANOSCALE | - |
dc.identifier.doi | 10.1039/c7nr09542b | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Park, Ki Soo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DNASE-I | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | TYPHIMURIUM | - |
dc.subject.keywordPlus | MARKER | - |
dc.subject.keywordPlus | PROBES | - |
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