DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Kwan Woo | ko |
dc.contributor.author | Lee, Chang Yeol | ko |
dc.contributor.author | Batule, Bhagwan S | ko |
dc.contributor.author | Park, Ki Soo | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2018-02-21T05:53:54Z | - |
dc.date.available | 2018-02-21T05:53:54Z | - |
dc.date.created | 2018-02-05 | - |
dc.date.created | 2018-02-05 | - |
dc.date.created | 2018-02-05 | - |
dc.date.created | 2018-02-05 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | RSC ADVANCES, v.8, no.4, pp.1958 - 1962 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | http://hdl.handle.net/10203/240162 | - |
dc.description.abstract | We describe a novel strategy for the ultrasensitive detection of target DNA based on rolling circle amplification (RCA) coupled with fluorescent poly(thymine)-templated copper nanoparticles (poly T-CuNPs). In the presence of target DNA, a padlock DNA probe that consists of two regions: a target DNA-specific region and a poly(adenine) region, is circularized by the ligation reaction, and the subsequent RCA reaction is promoted to generate long, concatemeric, single-stranded DNA (ssDNA) with a lot of repetitive poly T sequences. As a result, a large number of poly T-CuNPs are formed, exhibiting a highly fluorescent signal. However, in the absence of target DNA or in the presence of non-specific target DNA, the padlock DNA probe is not circularized and the subsequent RCA is not executed, leading to no production of fluorescent poly T-CuNPs. With this simple strategy, we successfully analyzed the target DNA with the ultralow detection limit of 7.79 aM, a value that is 3 or 7 orders of magnitude lower than those of previous RCA-based fluorescent DNA detection strategies. In addition, the developed system was demonstrated to selectively discriminate non-specific target DNAs with one-base mismatch, suggesting potential application in the accurate diagnosis of single nucleotide polymorphisms or mutations. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Ultrasensitive DNA detection based on target-triggered rolling circle amplification and fluorescent poly(thymine)-templated copper nanoparticles | - |
dc.type | Article | - |
dc.identifier.wosid | 000422863900032 | - |
dc.identifier.scopusid | 2-s2.0-85040962864 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 1958 | - |
dc.citation.endingpage | 1962 | - |
dc.citation.publicationname | RSC ADVANCES | - |
dc.identifier.doi | 10.1039/c7ra11071e | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Park, Ki Soo | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CASCADE SIGNAL AMPLIFICATION | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | QUANTITATIVE PCR | - |
dc.subject.keywordPlus | ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | TECHNOLOGIES | - |
dc.subject.keywordPlus | CASCADE SIGNAL AMPLIFICATION | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | QUANTITATIVE PCR | - |
dc.subject.keywordPlus | ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | TECHNOLOGIES | - |
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