Radiolytic immobilization of lipase on poly(glycidyl methacrylate)-grafted polyethylene microbeads

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dc.contributor.authorChoi, SHko
dc.contributor.authorLee, KPko
dc.contributor.authorKang, HDko
dc.contributor.authorPark, Hyun Gyuko
dc.date.accessioned2011-05-20T07:56:41Z-
dc.date.available2011-05-20T07:56:41Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2004-12-
dc.identifier.citationMACROMOLECULAR RESEARCH, v.12, no.6, pp.586 - 592-
dc.identifier.issn1598-5032-
dc.identifier.urihttp://hdl.handle.net/10203/23806-
dc.description.abstractPoly(glycidyl methacrylate)-grafted polyethylene microbeads (PGPM) presenting epoxy groups were prepared by radiation-induced graft polymerization of glycidyl methacrylate on the polyethylene microbead. The obtained PGPM was characterized by IR spectroscopic, X-ray photoelectrons spectroscopy (XPS), scanning electron microscope (SEM), and thermal analyses. Furthermore, the abundance of epoxy groups on the PGPM was determined by titration and elemental analysis after animation. The epoxy group content was calculated to be in the range 0.29similar to0.34 mmol/g when using the titration method, but in the range 0.53similar to0.59 mmol/g when using elemental analysis (EA) after animation. The lipase was immobilized to the epoxy groups of the PGPM under various experimental conditions, including changes to the pH and the epoxy group content. The activity of the lipase-immobilized PGPM was in the range from 160 to 500 unit/mg(.)min. The activity of the lipase-immobilized PGPM increased upon increasing the epoxy group content. The lipase-immobilized PGPM was characterized additionally by SEM, electron spectroscopy for chemical analysis (ESCA), and EA.-
dc.description.sponsorshipThe work was supported by the Science Foundation of Hannam University (2004).en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherPOLYMER SOC KOREA-
dc.subjectINDUCED GRAFT-COPOLYMERIZATION-
dc.subjectACID GROUP-
dc.subjectADSORPTION-
dc.subjectMEMBRANE-
dc.subjectFILM-
dc.subjectPOLYMERIZATION-
dc.subjectADSORBENTS-
dc.subjectAMIDOXIME-
dc.subjectUROKINASE-
dc.subjectAMINE-
dc.titleRadiolytic immobilization of lipase on poly(glycidyl methacrylate)-grafted polyethylene microbeads-
dc.typeArticle-
dc.identifier.wosid000226111000007-
dc.identifier.scopusid2-s2.0-12244311929-
dc.type.rimsART-
dc.citation.volume12-
dc.citation.issue6-
dc.citation.beginningpage586-
dc.citation.endingpage592-
dc.citation.publicationnameMACROMOLECULAR RESEARCH-
dc.identifier.doi10.1007/BF03218448-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorPark, Hyun Gyu-
dc.contributor.nonIdAuthorChoi, SH-
dc.contributor.nonIdAuthorLee, KP-
dc.contributor.nonIdAuthorKang, HD-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorepoxy group-
dc.subject.keywordAuthorglycidyl methacrylate-
dc.subject.keywordAuthorimmobilization-
dc.subject.keywordAuthorlipase-
dc.subject.keywordAuthorlipase activity-
dc.subject.keywordAuthorpoly(glycidyl methacrylate)-
dc.subject.keywordAuthorradiation-induced graft polymerization-
dc.subject.keywordPlusINDUCED GRAFT-COPOLYMERIZATION-
dc.subject.keywordPlusACID GROUP-
dc.subject.keywordPlusADSORPTION-
dc.subject.keywordPlusMEMBRANE-
dc.subject.keywordPlusFILM-
dc.subject.keywordPlusPOLYMERIZATION-
dc.subject.keywordPlusADSORBENTS-
dc.subject.keywordPlusAMIDOXIME-
dc.subject.keywordPlusUROKINASE-
dc.subject.keywordPlusAMINE-
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