In vitro and in vivo investigation for biological activities of neoagarooligosaccharides prepared by hydrolyzing agar with beta-agarase

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Our study investigated the biological functions of three neoagarooligosaccharides (NAOs), neoagarobiose (NA2), neoagarotetraose (NA4), and neoagarohexaose (NA6). NAOs were prepared by hydrolyzing agar with the recombinant beta-agarases, DagA and DagB, from Streptomyces coelicolor A3(2). An in vitro DPPH (diphenyl-2,4,6-trinitrophenyliminoazanium) radical scavenging assay revealed that none of the NAOs had any significant antioxidative activity. We also assessed the antibacterial activity of each NAO against several gram-negative and gram-positive bacteria. This revealed weak effects against four of the seven tested strains. NAO treatment resulted in significant inhibition of alpha-glucosidase, with the strongest effect observed in a NA4 and NA6 mixture, and a decreasing effect was observed in the order NA2 > NA4 > NA6. Little inhibition was observed against alpha-amylase, with an effect significantly lower than that of acarbose. No observable cytotoxicity was found in immortalized Melan-a mouse melanocytes by NAO treatment up to a concentration of 2,000 mu g/mL. However, melanin synthesis was significantly reduced by NAO treatment, with the strongest effect observed in a NA4 and NA6 mixture and decreasing effects observed in the order NA6 > NA4 > NA2. The tyrosinase activity of Melan-a cells was also consistently decreased by NAO treatment. Among the tested factors, treatment of NA4 and NA6 mixture showed the highest inhibition effects against alpha-glucosidase and tyrosinase, and melanin biosynthesis. Further in vivo study to investigate the role of NAO in these biological functions will be necessary for future biotechnological applications.
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Issue Date
2017-08
Language
English
Article Type
Article
Keywords

PURIFICATION; OLIGOSACCHARIDES; NEOAGAROBIOSE; MELANOMA

Citation

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.22, no.4, pp.489 - 496

ISSN
1226-8372
DOI
10.1007/s12257-017-0049-8
URI
http://hdl.handle.net/10203/226462
Appears in Collection
CBE-Journal Papers(저널논문)
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