Metabolic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli

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Poly(3-hydroxybutyrate) (PHB) production by fermentation was examined under both restricted- and ample-oxygen supply conditions in a single fed-batch fermentation. Recombinant Escherichia coli transformed with the PHB production plasmid pSYL107 was grown to reach high cell density (227 g/l dry cell weight) with a high PHB content (78% of dry cell weight), using a glucose-based minimal medium. A simple flux model containing 12 fluxes was developed and applied to the fermentation data. A superior closure (95%) of the carbon mass balance was achieved. When the data were put into use, the results demonstrated a surprisingly large excretion of formate and lactate. Even though periods of severe oxygen limitation coincided with rapid acetate and lactate excretion, PHB productivity and carbon utilization efficiency were not significantly impaired. These results are very positive in reducing oxygen demand in an industrial PHA fermentation without sacrificing its PHA productivity, thereby reducing overall production costs.
Publisher
SPRINGER-VERLAG SINGAPORE PTE LTD
Issue Date
1999-10
Language
English
Article Type
Article
Keywords

BIOCHEMICAL REACTION SYSTEMS; LINEAR CONSTRAINT RELATIONS; FLUX ANALYSIS; MASS BALANCES; CAPABILITIES; CULTURE; GROWTH; POLYHYDROXYALKANOATES; OVERPRODUCTION; DISTRIBUTIONS

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.9, no.5, pp.593 - 603

ISSN
1017-7825
URI
http://hdl.handle.net/10203/22623
Appears in Collection
CBE-Journal Papers(저널논문)
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