Bioprocess engineering to produce 9-(nonanoyloxy) nonanoic acid by a recombinant Corynebacterium glutamicum-based biocatalyst

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Here, Corynebacterium glutamicum ATCC13032 expressing Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. Diverse parameters including cultivation and reaction temperatures, type of detergent, and pH were found to improve biotransformation efficiency. The optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant C. glutamicum was 15 A degrees C, but the reaction temperature was optimal at 30 A degrees C. Enhanced conversion efficiency was obtained by supplying 0.05 g/L of Tween 80 at pH 7.5. Under these optimal conditions, recombinant C. glutamicum produced 0.28 mM of 9-(nonanoyloxy) nonanoic acid with a 75.6% (mol/mol) conversion yield in 2 h. This is the first report on the biotransformation of 10-ketostearic acid to 9-(nonanoyloxy) nonanoic acid with a recombinant whole-cell C. glutamicum-based biocatalyst and the results demonstrate the feasibility of using C. glutamicum as a whole-cell biocatalyst.
Publisher
SPRINGER HEIDELBERG
Issue Date
2017-09
Language
English
Article Type
Article
Citation

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.44, no.9, pp.1301 - 1311

ISSN
1367-5435
DOI
10.1007/s10295-017-1945-9
URI
http://hdl.handle.net/10203/225983
Appears in Collection
CBE-Journal Papers(저널논문)
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