DC Field | Value | Language |
---|---|---|
dc.contributor.author | Luo, Zi Wei | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2017-06-16T03:58:10Z | - |
dc.date.available | 2017-06-16T03:58:10Z | - |
dc.date.created | 2017-06-12 | - |
dc.date.created | 2017-06-12 | - |
dc.date.created | 2017-06-12 | - |
dc.date.created | 2017-06-12 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.citation | NATURE COMMUNICATIONS, v.8 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/10203/224058 | - |
dc.description.abstract | Terephthalic acid (TPA) is an important industrial chemical currently produced by energy intensive and potentially hazardous p-xylene (pX) oxidation process. Here we report the development of metabolically engineered Escherichia coli system for biological transformation of pX into TPA. The engineered E. coli strain harbours a synthetic TPA pathway optimized through manipulation of expression levels of upstream and downstream modules. The upstream pathway converts pX to p-toluic acid (pTA) and the downstream pathway transforms pTA to TPA. In a two-phase partitioning fermentation, the engineered strain converts 8.8 g pX into 13.3 g TPA, which corresponds to a conversion yield of 96.7 mol%. These results suggest that the E. coli system presented here might be a promising alternative for the large-scale biotechnological production of TPA and lays the foundations for the future development of sustainable approaches for TPA production. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Biotransformation of p-xylene into terephthalic acid by engineered Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000402395800001 | - |
dc.identifier.scopusid | 2-s2.0-85020018556 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.publicationname | NATURE COMMUNICATIONS | - |
dc.identifier.doi | 10.1038/ncomms15689 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | COMAMONAS-TESTOSTERONI T-2 | - |
dc.subject.keywordPlus | PSEUDOMONAS-PUTIDA | - |
dc.subject.keywordPlus | TOLUENESULFONATE | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | ALDEHYDES | - |
dc.subject.keywordPlus | TOLUENE | - |
dc.subject.keywordPlus | OPERON | - |
dc.subject.keywordPlus | GENES | - |
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