Controllable gold-capped nanoporous anodic alumina chip for label-free, specific detection of bacterial cells

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We describe the development of a gold-capped nanostructured porous anodic alumina (PAA) chip, which makes use of localized surface plasmon resonance and interferometry for the label-free and specific detection of pathogenic bacterial cells via changes in relative reflected intensity (RRI) and wavelength shift. This chip is composed of PAA and gold layers that provide a reproducible and sensitive RRI and wavelength shift. We investigated the effects of varying the thicknesses of the PAA and gold layers on the optical responses of the chip. With this chip, we successfully detected and identified Pseudomonas aeruginosa in a proof-of-concept experiment; this chip can detect bacterial cells in a quantitative manner with a detection limit of 20 CFU per assay. Further, this chip based system can clearly identify target bacterial species in a multiplexed mode with high specificities on a single chip, so will be useful for the detection of various pathogenic microbes.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2017
Language
English
Article Type
Article
Citation

RSC ADVANCES, v.7, no.30, pp.18815 - 18820

ISSN
2046-2069
DOI
10.1039/c6ra27130h
URI
http://hdl.handle.net/10203/223804
Appears in Collection
CBE-Journal Papers(저널논문)
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